Temperature stability of urinary F2-isoprostane and 8-hydroxy-2′-deoxyguanosine

IF 1.7 Q3 MEDICAL LABORATORY TECHNOLOGY

Practical Laboratory Medicine Pub Date : 2024-02-16 DOI:10.1016/j.plabm.2024.e00373

Katarzyna Kordas , Diala Ghazal , Elena I. Queirolo , James R. Olson , María Inés Beledo , Richard W. Browne

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Abstract

Background

Clinical and epidemiological studies employ long-term temperature storage but the effect of temperature on the stability of oxidative stress (OS) markers is unknown. We investigated the effects of storage at −20 °C and −80 °C over 4–9 months on F₂-isoprostanes (F₂-IsoP) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels in urine of children, a population group among whom the measurement of these markers is still limited.

Methods

Paired spot urine samples from 87 children aged 8.9–16.9 years (52.9% boys) were analyzed. Samples were preserved with 0.005% (w/v) butylated hydroxytoluene, portioned and stored within 2.5 h (median) of collection. Samples were analyzed in duplicate or triplicate using commercial ELISA kits and their correlations were evaluated.

Results

F₂-IsoP and 8-OHdG showed high correlations (Spearman rho of 0.90 and 0.97, respectively; P < 0.0001) with storage at −20 °C and −80 °C. There was a strong agreement among categories of values for F₂-IsoP (Kappa = 0.76 ± 0.08, agreement = 83.9%, P < 0.0001) and 8-OHdG: (Kappa = 0.83 ± 0.08, agreement = 88.4%, P < 0.0001). The correlation between the temperatures for F₂-IsoP concentrations was also high when stored for <4 (0.93), 4 (0.93), and 5 months (0.88), all P < 0.0001. For 8-OHdG, Spearman correlations at <8, 8, and 9 months of storage at −20 °C and −80 °C were 0.95, 0.98, and 0.96 (all P < 0.0001), respectively.

Conclusions

Urine storage with BHT for up to nine months at a temperature of −20 °C to −80 °C yields highly comparable concentrations of F₂-IsoP and 8-OHdG.

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尿液中 F2-异前列腺素和 8-羟基-2′-脱氧鸟苷的温度稳定性

背景临床和流行病学研究采用长期温度储存，但温度对氧化应激（OS）标志物稳定性的影响尚不清楚。我们研究了在-20 °C和-80 °C条件下储存4-9个月对儿童尿液中F2-异前列腺素（F2-IsoP）和8-羟基-2′-脱氧鸟苷（8-OHdG）水平的影响。样本用 0.005%（w/v）丁基羟基甲苯进行保存，并在采集后 2.5 小时（中位数）内分装和储存。结果F2-IsoP和8-OHdG与-20 °C和-80 °C的储存有很高的相关性（Spearman rho分别为0.90和0.97；P < 0.0001）。F2-IsoP（Kappa = 0.76 ± 0.08，吻合度 = 83.9%，P < 0.0001）和 8-OHdG：（Kappa = 0.83 ± 0.08，吻合度 = 88.4%，P < 0.0001）的各类值之间的吻合度很高。储存 4 个月（0.93）、4 个月（0.93）和 5 个月（0.88）时，F2-IsoP 浓度的温度之间的相关性也很高，均为 P <0.0001。对于 8-OHdG，在 -20 °C 和 -80 °C 下储存 8 个月、8 个月和 9 个月时的 Spearman 相关性分别为 0.95、0.98 和 0.96（均为 P <0.0001）。

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来源期刊

Practical Laboratory Medicine Health Professions-Radiological and Ultrasound Technology

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

7 weeks

期刊介绍： Practical Laboratory Medicine is a high-quality, peer-reviewed, international open-access journal publishing original research, new methods and critical evaluations, case reports and short papers in the fields of clinical chemistry and laboratory medicine. The objective of the journal is to provide practical information of immediate relevance to workers in clinical laboratories. The primary scope of the journal covers clinical chemistry, hematology, molecular biology and genetics relevant to laboratory medicine, microbiology, immunology, therapeutic drug monitoring and toxicology, laboratory management and informatics. We welcome papers which describe critical evaluations of biomarkers and their role in the diagnosis and treatment of clinically significant disease, validation of commercial and in-house IVD methods, method comparisons, interference reports, the development of new reagents and reference materials, reference range studies and regulatory compliance reports. Manuscripts describing the development of new methods applicable to laboratory medicine (including point-of-care testing) are particularly encouraged, even if preliminary or small scale.