Diversity of Glossinidae (Diptera) species in The Gambia in relation to vegetation.

IF 1.3 4区农林科学 Q2 Veterinary

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-02-19 eCollection Date: 2024-01-01 DOI:10.1590/S1984-29612024010

Alpha Kargbo, Mamudou Jallow, Thallitha Samih Wischral Jayme Vieira, Amien Isaac Amoutchi, Herve Koukoua Koua, Aamir Muse Osman, Rafael Felipe da Costa Vieira

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Abstract

Glossina species are known to transmit African Trypanosomiasis, one of the most important infectious diseases for both livestock and humans in sub-Saharan Africa. Therefore, the aim of this study was to characterize trapped Glossina spp. from The Gambia using morphological and molecular techniques in relation to the vegetation cover types. A line transect survey was carried out in all the administrative regions of The Gambia. Tsetse fly trapping was carried out for 14 days during each season using line transect. A total of 220 Glossina spp. specimens (117 F and 103 M) were captured, and DNA was extracted from the legs of 100 randomly selected Glossina spp. Further, DNA samples were tested by a conventional PCR assay. A total of 135/220 (61%; 95% CI: 54.6-67.8%) and 85/220 (39%; 95% CI: 32.2-45.4%) flies were identified as Glossina morsitans submorsitans and Glossina palpalis gambiensis, respectively, with most caught during wet season (53.6%) and more females (53.2%) than males. Results of the morphological identification agreed with those of molecular identification. The type of vegetation cover significantly influenced the caught of tsetse flies. Animals and humans at the various trapping sites are at risk of being bitten by tsetse flies.

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冈比亚 Glossinidae（双翅目）物种多样性与植被的关系。

据了解，非洲锥虫病是撒哈拉以南非洲地区牲畜和人类最重要的传染病之一，Glossina种可传播非洲锥虫病。因此，本研究的目的是利用形态学和分子技术，结合植被覆盖类型，描述冈比亚被困 Glossina 的特征。研究人员在冈比亚所有行政区域进行了横断面调查。在每个季节使用线状横断面进行了为期 14 天的采采蝇诱捕。共捕获了 220 个 Glossina 种标本（117 个 F 和 103 个 M），并从随机抽取的 100 个 Glossina 种标本的腿部提取了 DNA。此外，还采用传统的 PCR 方法对 DNA 样本进行了检测。结果表明，135只/220只（61%；95% CI：54.6-67.8%）和85只/220只（39%；95% CI：32.2-45.4%）苍蝇分别被鉴定为Glossina morsitans submorsitans和Glossina palpalis gambiensis，其中大部分在雨季捕获（53.6%），雌蝇（53.2%）多于雄蝇。形态鉴定结果与分子鉴定结果一致。植被类型对采采蝇的捕获有很大影响。在不同的诱捕地点，动物和人类都有被采采蝇叮咬的风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Brasileira De Parasitologia Veterinaria PARASITOLOGY-VETERINARY SCIENCES

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： La revista es un órgano de difusión del Colegio Brasileño de Parasitología Veterinaria, con una especificidad dentro de esa área, la difusión de los resultados de la investigación brasileña en las áreas de Helmintología, Protozoología, Entomología y agentes transmitidos por artrópodos, relacionados con la salud animal.