Follicular fluid concentration of soluble Human-G Leukocytic Antigen (sHLA-G) in in vitro fertilization cycles of women with and without peritoneal endometriosis.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Glícia Pinheiro Bezerra, Vanesa K Genro, Carlos Augusto B Souza, João Sabino Cunha-Filho
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Abstract

Objective: The objective of this research is to investigate the association between the concentrations of soluble human leukocyte G antigen (sHLA-G) in the follicular fluid (FF) in infertile patients with peritoneal endometriosis submitted to in vitro fertilization.

Methods: We performed a cross-sectional study, including ninety-six women undergoing in vitro fertilization (IVF) ageing ≤ 40 years. Infertile patients were classified into two groups: with endometriosis diagnosed by laparoscopy and without endometriosis due to tubal factor. ELISA measured soluble HLA-G in the FF of a pool of punctured (more than 17mm) follicles from women with endometriosis and without endometriosis who were subjected to ovulation induction for IVF. Embryos obtained after fertilization were classified according to the graduated embryo score (GES).

Results: Groups were comparables in terms of age, the number of follicles, AMH, FSH and all included reproductive outcomes. There was no association between sHLA-G concentrations and the average score of the generated embryos (p>0.05). Measurement of sHLA-G in the follicle fluid in women with endometriosis and without endometriosis (tubal factor) showed no significant difference (p>0.05). We also compared sHLA-G per follicle and per embryo, which were not different between both groups (p>0.05).

Conclusions: Patients with peritoneal endometriosis submitted to IVF did not demonstrate an altered sHLA-G in the follicular fluid compared to the follicular fluid sHLA-G concentration in tubal factor patients. Also, this molecule was not linked to any other reproductive outcome.

患有和未患有腹膜子宫内膜异位症的妇女体外受精周期中卵泡液中可溶性人类-G 白细胞抗原(sHLA-G)的浓度。
研究目的本研究旨在探讨接受体外受精的腹膜子宫内膜异位症不孕患者卵泡液(FF)中可溶性人类白细胞G抗原(sHLA-G)浓度之间的关联:我们进行了一项横断面研究,其中包括96名年龄小于40岁的体外受精(IVF)女性。不孕患者被分为两组:经腹腔镜检查确诊患有子宫内膜异位症和因输卵管因素导致的无子宫内膜异位症。酶联免疫吸附法测定了子宫内膜异位症和无子宫内膜异位症妇女的穿刺卵泡(大于 17 毫米)FF 中的可溶性 HLA-G,这些妇女都接受了体外受精的促排卵治疗。受精后获得的胚胎根据胚胎分级评分(GES)进行分类:各组在年龄、卵泡数量、AMH、FSH和所有生殖结果方面具有可比性。sHLA-G 浓度与生成胚胎的平均得分之间没有关联(P>0.05)。对患有子宫内膜异位症和不患有子宫内膜异位症(输卵管因素)的妇女的卵泡液中的 sHLA-G 进行测量,结果显示两者没有显著差异(p>0.05)。我们还比较了每个卵泡和每个胚胎的 sHLA-G,两组之间没有差异(P>0.05):结论:与输卵管因素患者卵泡液中的sHLA-G浓度相比,接受体外受精的腹膜子宫内膜异位症患者卵泡液中的sHLA-G并未发生变化。此外,该分子与其他生殖结果也没有关联。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.30
自引率
6.70%
发文量
56
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