Evaluation of Pendrin Expression Using Nuclear Imaging Modalities and Immunohistochemistry in Animal Thyroid Cancer Model.

IF 0.4 Q4 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Indian Journal of Nuclear Medicine Pub Date : 2023-10-01 Epub Date: 2023-12-20 DOI:10.4103/ijnm.ijnm_46_23
Chandrakala Sanjay Gholve, Yogita Shete, Sutapa Rakshit, Savita Kulkarni
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引用次数: 0

Abstract

Context: The impaired ability of thyroid cancer (TC) cells to uptake and concentrate iodine represents a major therapeutic challenge in malignant TC management. This has been reported probably due to reduced or loss of expression of pendrin in thyroid tumors.

Aims: In view of this, we evaluated the pendrin expression in the chemically induced (using N-bis[2-hydroxypropyl] nitrosamine [DHPN]) TC model in Wistar rats.

Methods: Uptake in the thyroid gland was evaluated by positron emission tomography with computed tomography (PET-CT) and scintigraphy imaging. Further histopathology (HP) and immunohistochemistry (IHC) were performed for confirming malignancy.

Results: The altered uptake in the thyroid gland was observed by PET-CT and scintigraphy imaging. Significant pathological changes in the thyroid were observed using 2-deoxy-2-(fluorine-18) fluoro-D-glucose PET-CT, technetium-99m pertechnetate imaging, and reduced iodine-131 uptake (n = 4) in DHPN-induced animals compared to control indicative of thyroid cell proliferation. In treated groups, tissue HP revealed hyperplastic follicular to papillary cell proliferation with variable mitotic activity. The malignant nature of the tissue and variable uptake of the tracer were further reconfirmed by IHC. IHC revealed reduced pendrin expression in malignant thyroid tissue.

Conclusions: Hence, nuclear imaging techniques can be of aid in the early identification and evaluation of cellular changes during the early development of tumor models in laboratory animals. In conclusion, our study reveals that pendrin expression plays a vital role in thyroid uptake, and its reduction was observed in TC in a chemically induced TC model.

在甲状腺癌动物模型中使用核成像模式和免疫组化方法评估 Pendrin 的表达。
背景:甲状腺癌(TC)细胞吸收和浓缩碘的能力受损是恶性甲状腺癌治疗过程中的一大挑战。目的:有鉴于此,我们评估了化学诱导(使用 N-双[2-羟基丙基]亚硝胺 [DHPN])Wistar 大鼠甲状腺癌模型中 pendrin 的表达情况:方法:通过正电子发射计算机断层扫描(PET-CT)和闪烁扫描成像评估甲状腺摄取量。进一步进行组织病理学(HP)和免疫组织化学(IHC)检查以确认恶性肿瘤:结果:PET-CT 和闪烁扫描成像可观察到甲状腺摄取量的改变。与对照组相比,使用2-脱氧-2-(氟-18)氟-D-葡萄糖PET-CT、锝-99m过硫酸盐成像和碘-131摄取量减少(n = 4)可观察到甲状腺的显著病理变化,表明甲状腺细胞增生。在治疗组中,组织HP显示增生的滤泡状至乳头状细胞增生,有丝分裂活性不一。IHC进一步证实了组织的恶性和示踪剂的不同摄取。IHC显示恶性甲状腺组织中pendrin表达减少:因此,核成像技术有助于早期识别和评估实验动物肿瘤模型早期发展过程中的细胞变化。总之,我们的研究揭示了垂体促甲状腺激素表达在甲状腺摄取中的重要作用,并且在化学诱导的甲状腺肿模型中观察到垂体促甲状腺激素表达减少。
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来源期刊
Indian Journal of Nuclear Medicine
Indian Journal of Nuclear Medicine RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
0.70
自引率
0.00%
发文量
46
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