Exogenous application of pectin triggers stomatal closure and immunity in Arabidopsis.

IF 4.8 1区农林科学 Q1 PLANT SCIENCES

Molecular plant pathology Pub Date : 2024-02-01 DOI:10.1111/mpp.13438

Cheng Zhang, Charles Tetteh, Sheng Luo, Pinyuan Jin, Xingqian Hao, Min Sun, Nan Fang, Yingjun Liu, Huajian Zhang

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引用次数: 0

Abstract

Pectin has been extensively studied in animal immunity, and exogenous pectin as a food additive can provide protection against inflammatory bowel disease. However, the utility of pectin to improve immunity in plants is still unstudied. Here, we found exogenous application of pectin triggered stomatal closure in Arabidopsis in a dose- and time-dependent manner. Additionally, pectin activated peroxidase and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase to produce reactive oxygen species (ROS), which subsequently increased cytoplasmic Ca²⁺ concentration ([Ca²⁺ ]_cyt ) and was followed by nitric oxide (NO) production, leading to stomatal closure in an abscisic acid (ABA) and salicylic acid (SA) signalling-dependent mechanism. Furthermore, pectin enhanced the disease resistance to Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) with mitogen-activated protein kinases (MPKs) MPK3/6 activated and upregulated expression of defence-responsive genes in Arabidopsis. These results suggested that exogenous pectin-induced stomatal closure was associated with ROS and NO production regulated by ABA and SA signalling, contributing to defence against Pst DC3000 in Arabidopsis.

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外源应用果胶可引发拟南芥气孔关闭和免疫。

果胶在动物免疫方面已得到广泛研究，作为食品添加剂的外源果胶可提供对炎症性肠病的保护。然而，果胶在提高植物免疫力方面的作用仍未得到研究。在这里，我们发现外源果胶以剂量和时间依赖的方式引发拟南芥气孔关闭。此外，果胶激活过氧化物酶和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶，产生活性氧（ROS），进而增加细胞质 Ca2+ 浓度（[Ca2+ ]cyt ），随后产生一氧化氮（NO），在脱落酸（ABA）和水杨酸（SA）信号依赖机制下导致气孔关闭。此外，果胶通过激活有丝分裂原激活蛋白激酶（MPKs）MPK3/6，并上调拟南芥防御响应基因的表达，增强了拟南芥对西红柿假单胞菌病菌 DC3000（Pst DC3000）的抗病性。这些结果表明，外源果胶诱导的气孔关闭与受 ABA 和 SA 信号调控的 ROS 和 NO 生成有关，有助于拟南芥抵御 Pst DC3000。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular plant pathology 生物-植物科学

CiteScore

9.40

自引率

4.10%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.