Different profiles of chemokines, cytokines and cell growth factors in plasma samples from patients with leprosy, leprosy reactions and households contacts.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-02-19 eCollection Date: 2024-01-01 DOI:10.1590/0074-02760230129

Jairo Campos de Carvalho, Marcelo Antônio Pascoal-Xavier, Marcelo Grossi Araújo, Andrea Teixeira-Carvalho, Olindo Assis Martins-Filho, Vanessa Peruhype-Magalhães, Jordana Grazziela Alves Coelho-Dos-Reis, Márcio Sobreira Silva Araújo

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引用次数: 0

Abstract

Background: Leprosy is a highly neglected disease that is considered a serious public health problem in many countries. This illness is characterised by a variety of clinical and histopathological manifestations that are related to the patient immune response.

Objectives: This work aimed evaluate the profile of circulating immune mediators in the plasma from patients classified clinically as paucibacillary (PB), multibacillary (MB), households contacts (HHC), type1 leprosy reaction (T1R), type2 leprosy reaction (T2R) and control individuals without medical history of leprosy (CTL).

Methods: To assessment of the plasma immune mediators was used multiplex microbeads immunoassay "Luminex".

Findings: The results showed that patients (PB) had a regulatory-biased profile, while MB revealed a pro-inflammatory trend of highly expressed biomarkers. HHC display conspicuously increased levels in the plasma of the chemokines (CCL2, CCL3, CCL4, CCL5 and CXCL8), pro-inflammatory cytokines (IFN-γ,TNF and IL-1β), modulating cytokines (IL-9 and IL-1Ra) and growth factors (PDGF, G-CSF and IL-2). Interestingly, HHC displayed superior production of IFN-γ as compared to other leprosy groups, indicating a putative protective role for this cytokine during chronic Mycobacterium leprae exposure.

Main conclusion: Further investigations are currently underway to elucidate the potential of these mediators as biomarkers applicable to the diagnosis/prognosis of leprosy and also T1R and T2R leprosy reactions.

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麻风病人、麻风反应者和家庭接触者血浆样本中趋化因子、细胞因子和细胞生长因子的不同特征。

背景：麻风病是一种极易被忽视的疾病，在许多国家被视为严重的公共卫生问题。这种疾病具有多种临床和组织病理学表现，与患者的免疫反应有关：本研究旨在评估临床分类为贫脓疱型（PB）、多脓疱型（MB）、家庭接触者（HHC）、1型麻风反应（T1R）、2型麻风反应（T2R）的患者以及无麻风病史的对照组（CTL）血浆中循环免疫介质的概况：方法：使用多重微珠免疫测定 "Luminex "来评估血浆免疫介质：结果：结果表明，患者（PB）具有调节偏倚的特征，而 MB 则显示出高表达生物标志物的促炎趋势。HHC 血浆中趋化因子（CCL2、CCL3、CCL4、CCL5 和 CXCL8）、促炎细胞因子（IFN-γ、TNF 和 IL-1β）、调节细胞因子（IL-9 和 IL-1Ra）和生长因子（PDGF、G-CSF 和 IL-2）的水平明显升高。有趣的是，与其他麻风病组相比，HHC 显示出更高的 IFN-γ 生成量，表明这种细胞因子在麻风分枝杆菌慢性暴露期间可能起保护作用：主要结论：目前正在开展进一步研究，以阐明这些介质作为生物标志物的潜力，这些生物标志物可用于麻风病的诊断/预后以及T1R和T2R麻风病反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464