Extraction of chlorophyll a from Tetradesmus obliquus-a method upgrade.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-01 Epub Date: 2024-02-22 DOI:10.1007/s42977-024-00209-3

E Greipel, A Kósa, B Böddi, M Bakony, G Bernát, T Felföldi, É Preininger, J Kutasi

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Abstract

Nowadays, the use of algae is prevalent for both industrial and agricultural purposes. The determination of chlorophyll (Chl) content is a commonly used method for estimating the phytoplankton abundance in different water bodies or biomass density of algal cultures. The aim of the present work is to optimise the efficiency of the Chl extraction from the green alga Tetradesmus obliquus using methanol as extracting solvent. The extraction efficiency was estimated by measuring the Chl a concentration of the extracts using fluorescence spectroscopy. To increase the extraction yield, glass fibre filters with algal cells on top were treated with 10% (v/v) formalin prior to the extraction. We found that this pretreatment significantly enhanced the extraction yield of Chl without its chemical decomposition. We also found that the optimal cell concentration for Chl determination ranged from 1.44 × 10⁴ to 3.60 × 10⁵ cells/mL and the extraction efficiency was lower when the cell density of the culture was out of this range. These results highlight the importance of the optimization of the pigment extraction for the studied algal species.

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从四裂殖藻中提取叶绿素 a 的方法升级。

如今，藻类在工业和农业方面的应用都很普遍。叶绿素（Chl）含量测定是估算不同水体中浮游植物丰度或藻类培养生物量密度的常用方法。本研究旨在优化以甲醇为提取溶剂从绿色藻类 Tetradesmus obliquus 中提取叶绿素的效率。萃取效率通过使用荧光光谱法测量萃取物中的 Chl a 浓度来估算。为了提高萃取率，在萃取前用 10%（v/v）福尔马林处理带有藻细胞的玻璃纤维过滤器。我们发现，这种预处理方法大大提高了叶绿素的提取率，而且不会发生化学分解。我们还发现，测定 Chl 的最佳细胞浓度范围为 1.44 × 104 至 3.60 × 105 cells/mL，当培养物的细胞密度超出此范围时，提取效率较低。这些结果凸显了对所研究的藻类物种进行色素提取优化的重要性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.