Effects of alcohol on the composition and metabolism of the intestinal microbiota among people with HIV: A cross-sectional study

IF 2.5 4区医学 Q3 PHARMACOLOGY & PHARMACY

Alcohol Pub Date : 2024-02-20 DOI:10.1016/j.alcohol.2024.02.003

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引用次数: 0

Abstract

Objectives

Alcohol consumption is not uncommon among people with HIV (PWH) and may exacerbate HIV-induced intestinal damage, and further lead to dysbiosis and increased intestinal permeability. This study aimed to determine the changes in the fecal microbiota and its association with alcohol consumption in HIV-infected patients.

Methods

A cross-sectional survey was conducted between November 2021 and May 2022, and 93 participants were recruited. To investigate the alterations of alcohol misuse on fecal microbiology in HIV-infected individuals, we performed 16s rDNA gene sequencing on fecal samples from the low-to-moderate drinking (n = 21) and non-drinking (n = 72) groups.

Results

Comparison between groups using alpha and beta diversity showed that the diversity of stool microbiota in the low-to-moderate drinking group did not differ from that of the non-drinking group (all p > 0.05). The Linear discriminant Analysis effect size (LEfSe) algorithm was used to determine the bacterial taxa associated with alcohol consumption, and the results showed altered fecal bacterial composition in HIV-infected patients who consumed alcohol; Coprobacillus, Pseudobutyrivibrio, and Peptostreptococcaceae were enriched, and Pasteurellaceae and Xanthomonadaceae were depleted. In addition, by using the Kyoto Encyclopedia of Genes and Genomes (KEGG), functional microbiome features were also found to be altered in the low-to-moderate drinking group compared to the control group, showing a reduction in metabolic pathways (p = 0.036) and cardiovascular disease pathways (p = 0.006).

Conclusion

Low-to-moderate drinking will change the composition, metabolism, and cardiovascular disease pathways of the gut microbiota of HIV-infected patients.

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酒精对艾滋病毒感染者肠道微生物群组成和代谢的影响：一项横断面研究

目的饮酒在艾滋病病毒感染者（PWH）中并不少见，可能会加剧艾滋病病毒引起的肠道损伤，并进一步导致菌群失调和肠道通透性增加。本研究旨在确定 HIV 感染者粪便微生物群的变化及其与饮酒的关系。为了研究酒精滥用对艾滋病病毒感染者粪便微生物的改变，我们对中低度饮酒组（n = 21）和不饮酒组（n = 72）的粪便样本进行了 16s rDNA 基因测序。结果使用阿尔法和贝塔多样性进行组间比较显示，中低度饮酒组粪便微生物群的多样性与不饮酒组没有差异（所有 p > 0.05）。利用线性判别分析效应大小（LEfSe）算法确定了与饮酒相关的细菌类群，结果显示饮酒的艾滋病感染者粪便细菌组成发生了改变；富集了铜绿假单胞菌、假布氏假单胞菌和胨链球菌科细菌，减少了巴斯德菌科和黄单胞菌科细菌。此外，通过使用《京都基因与基因组百科全书》（KEGG），还发现与对照组相比，中低度饮酒组的微生物组功能特征也发生了改变，代谢途径（p = 0.036）和心血管疾病途径（p = 0.006）均有所减少。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Alcohol 医学-毒理学

CiteScore

4.60

自引率

4.30%

发文量

审稿时长

15.6 weeks

期刊介绍： Alcohol is an international, peer-reviewed journal that is devoted to publishing multi-disciplinary biomedical research on all aspects of the actions or effects of alcohol on the nervous system or on other organ systems. Emphasis is given to studies into the causes and consequences of alcohol abuse and alcoholism, and biomedical aspects of diagnosis, etiology, treatment or prevention of alcohol-related health effects. Intended for both research scientists and practicing clinicians, the journal publishes original research on the neurobiological, neurobehavioral, and pathophysiological processes associated with alcohol drinking, alcohol abuse, alcohol-seeking behavior, tolerance, dependence, withdrawal, protracted abstinence, and relapse. In addition, the journal reports studies on the effects alcohol on brain mechanisms of neuroplasticity over the life span, biological factors associated with adolescent alcohol abuse, pharmacotherapeutic strategies in the treatment of alcoholism, biological and biochemical markers of alcohol abuse and alcoholism, pathological effects of uncontrolled drinking, biomedical and molecular factors in the effects on liver, immune system, and other organ systems, and biomedical aspects of fetal alcohol spectrum disorder including mechanisms of damage, diagnosis and early detection, treatment, and prevention. Articles are published from all levels of biomedical inquiry, including the following: molecular and cellular studies of alcohol''s actions in vitro and in vivo; animal model studies of genetic, pharmacological, behavioral, developmental or pathophysiological aspects of alcohol; human studies of genetic, behavioral, cognitive, neuroimaging, or pathological aspects of alcohol drinking; clinical studies of diagnosis (including dual diagnosis), treatment, prevention, and epidemiology. The journal will publish 9 issues per year; the accepted abbreviation for Alcohol for bibliographic citation is Alcohol.