PbrCSP1, a pollen tube–specific cold shock domain protein, is essential for the growth and cold resistance of pear pollen tubes

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2024-02-20 DOI:10.1007/s11032-024-01457-w

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引用次数: 0

Abstract

Cold shock domain proteins (CSPs), initially identified in Escherichia coli, have been demonstrated to play a positive role in cold resistance. Previous studies in wheat, rice, and Arabidopsis have indicated the functional conservation of CSPs in cold resistance between bacteria and higher plants. However, the biological functions of PbrCSPs in pear pollen tubes, which represent the fragile reproductive organs highly sensitive to low temperature, remain largely unknown. In this study, a total of 22 CSPs were identified in the seven Rosaceae species, with a focus on characterizing four PbrCSPs in pear (Pyrus bretschneideri Rehder). All four PbrCSPs were structurally conserved and responsive to the abiotic stresses, such as cold, high osmotic, and abscisic acid (ABA) treatments. PbrCSP1, which is specifically expressed in pear pollen tubes, was selected for further research. PbrCSP1 was localized in both the cytoplasm and nucleus. Suppressing the expression of PbrCSP1 significantly inhibited the pollen tube growth in vitro. Conversely, overexpression of PbrCSP1 promoted the growth of pear pollen tubes under the normal condition and, notably, under the cold environment at 4 °C. These findings highlight an essential role of PbrCSP1 in facilitating the normal growth and enhancing cold resistance in pear pollen tubes.

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梨花粉管特异性冷休克结构域蛋白 PbrCSP1 对梨花粉管的生长和抗寒性至关重要

摘要最初在大肠杆菌中发现的冷休克结构域蛋白（CSPs）已被证明在抗寒中发挥了积极作用。此前在小麦、水稻和拟南芥中进行的研究表明，CSPs 在细菌和高等植物的抗寒性中具有功能保护。然而，梨花粉管是对低温高度敏感的脆弱生殖器官，PbrCSPs 在梨花粉管中的生物学功能在很大程度上仍然未知。本研究在七种蔷薇科植物中共鉴定了 22 个 CSPs，重点研究了梨（Pyrus bretschneideri Rehder）中 4 个 PbrCSPs 的特征。所有四个 PbrCSP 在结构上都是保守的，并且对非生物胁迫，如低温、高渗透压和脱落酸（ABA）处理有反应。梨花粉管中特异表达的 PbrCSP1 被选作进一步研究的对象。PbrCSP1 定位于细胞质和细胞核中。抑制 PbrCSP1 的表达会明显抑制花粉管在体外的生长。相反，过表达 PbrCSP1 能促进梨花粉管在正常条件下的生长，尤其是在 4 °C 的低温环境下。这些发现凸显了 PbrCSP1 在促进梨花粉管正常生长和增强抗寒性方面的重要作用。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.