Spaceflight impacts xyloglucan oligosaccharide abundance in Arabidopsis thaliana root cell walls

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Xizheng Diao , Natasha Haveman , Brandon Califar , Xiaoru Dong , Boone Prentice , Anna-Lisa Paul , Robert J. Ferl
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Abstract

Over the course of more than a decade, space biology investigations have consistently indicated that cell wall remodeling occurs in a variety of spaceflight-grown plants. Here, we describe a mass spectrometric method to study the fundamental composition of xyloglucan, the most abundant hemicellulose in dicot cell walls, in space-grown plants. Four representative Arabidopsis root samples, from a previously conducted spaceflight experiment - Advanced Plant EXperiment - 04 (APEX-04), were used to investigate changes in xyloglucan oligosaccharides abundances in spaceflight-grown plants compared to ground controls. In situ localized enzymatic digestions and surface sampling mass spectrometry analysis provided spatial resolution of the changes in xyloglucan oligosaccharides abundances. Overall, the results showed that oligosaccharide XXLG/XLXG and XXFG branching patterns were more abundant in the lateral roots of spaceflight-grown plants, while XXXG, XLFG, and XLFG/XLFG were more abundant in the lateral roots of ground control plants. In the primary roots, XXFG had a higher abundance in ground controls than in spaceflight plants. This methodology of analyzing the basic components of the cell wall in this paper highlights two important findings. First, that are differences in the composition of xyloglucan oligosaccharides in spaceflight root cell walls compared to ground controls and, second, most of these differences are observed in the lateral roots. Thus, the methodology described in this paper provides insights into spaceflight cell wall modifications for future investigations.

太空飞行影响拟南芥根细胞壁中木糖寡聚糖的丰度
十多年来,太空生物学研究一直表明,细胞壁重塑发生在各种太空飞行生长的植物中。在这里,我们描述了一种质谱方法,用于研究太空生长植物中木糖的基本成分,木糖是双子叶植物细胞壁中最丰富的半纤维素。我们利用之前进行的一项太空飞行实验--高级植物实验-04(APEX-04)--中的四个具有代表性的拟南芥根样本,研究了与地面对照组相比,太空飞行生长植物中木糖寡糖丰度的变化。原位局部酶解和表面取样质谱分析提供了木聚糖低聚糖丰度变化的空间分辨率。总之,研究结果表明,在太空种植植物的侧根中,XXLG/XLXG 和 XXFG 分支模式的寡糖含量更高,而在地面对照植物的侧根中,XXXG、XLFG 和 XLFG/XLFG 的含量更高。在主根中,XXFG 在地面对照植物中的含量高于太空飞行植物。本文分析细胞壁基本成分的方法突出了两个重要发现。首先,与地面对照相比,太空飞行根细胞壁中木糖寡糖的组成存在差异;其次,这些差异大多出现在侧根中。因此,本文介绍的方法为今后的研究提供了有关太空飞行细胞壁变化的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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