Comparison of conjunctival swabs and blood samples in dogs for molecular and serological detection of Leishmania infantum infection in Colombia.

IF 0.8 4区医学 Q4 INFECTIOUS DISEASES

Journal of Vector Borne Diseases Pub Date : 2024-07-01 Epub Date: 2024-09-21 DOI:10.4103/JVBD.JVBD_182_23

Wilmer A Mejía-Chimá, Samuel D Hernández-Vera, Oscar Y Pérez-Vargas, Matilde E Rivero Rodriguez, E Paternina Luis, E Bejarano Eduar

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引用次数: 0

Abstract

Background objectives: Surveillance of canine leishmaniasis in Colombia is restricted to the appearance of visceral leishmaniasis cases in humans, and is mainly performed by serological tests. This requires blood sampling by veterinarians or technicians according to Colombian laws. The aim of this study was to evaluate the utility of conjunctival swabs in the molecular detection of Leishmania in dogs from the municipality of Ovejas, Sucre.

Methods: The present study was cross-sectional and descriptive. The collection source of samples and information was primary. Blood samples and conjunctival swabs from 121 dogs were analysed by PCR-ITS1 to detect Leishmania spp. Positive samples were used to amplify a conserved region of the Leishmania infantum kinetoplast minicircle. Performance of both sample types was calculated by proportion of positive samples of each type and the degree of agreement between them was determined by Cohen's kappa (κ) agreement index.

Results: Leishmania infection was detected in 17.4% (21/121) of blood samples and in 16.5% (20/121) of conjunctival swabs. In total, 28.1% (34/121) of the canines were infected, of which 11.8% (4/34) were infected with L. infantum in the conjunctival swabs and 5.9 % (2/34) in the blood samples. The agreement between blood and conjunctiva was medium (κ = 0.207) by PCR-ITS1 amplification.

Interpretation conclusion: The use of conjunctival swab as a non-invasive sample could be used as an alternative method for surveillance of canine leishmaniasis.

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比较哥伦比亚犬结膜拭子和血液样本对婴儿利什曼原虫感染的分子和血清学检测。

背景目标：哥伦比亚对犬类利什曼病的监控仅限于人类出现内脏利什曼病病例，主要通过血清学检测进行。根据哥伦比亚法律，这需要兽医或技术人员进行血液采样。本研究的目的是评估结膜拭子在分子检测苏克雷省奥韦哈斯市狗体内利什曼原虫的实用性：本研究为横断面描述性研究。样本和信息的收集来源是主要的。通过 PCR-ITS1 对 121 只狗的血样和结膜拭子进行分析，以检测利什曼原虫属。两种样本类型的检测结果按每种类型的阳性样本比例计算，两者之间的一致程度按科恩卡帕（κ）一致指数确定：结果：17.4%（21/121）的血液样本和 16.5%（20/121）的结膜拭子中检测到利什曼原虫感染。总共有 28.1%（34/121）的犬感染了利什曼原虫，其中 11.8%（4/34）的犬在结膜拭子中感染了幼犬利什曼原虫，5.9%（2/34）的犬在血液样本中感染了幼犬利什曼原虫。通过 PCR-ITS1 扩增，血液和结膜之间的一致性为中等（κ = 0.207）：使用结膜拭子作为非侵入性样本可作为监测犬利什曼病的替代方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Vector Borne Diseases INFECTIOUS DISEASES-PARASITOLOGY

CiteScore

0.90

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： National Institute of Malaria Research on behalf of Indian Council of Medical Research (ICMR) publishes the Journal of Vector Borne Diseases. This Journal was earlier published as the Indian Journal of Malariology, a peer reviewed and open access biomedical journal in the field of vector borne diseases. The Journal publishes review articles, original research articles, short research communications, case reports of prime importance, letters to the editor in the field of vector borne diseases and their control.