Development of LC-MS/MS method for cyanoenone triterpenoid determination to support CNS tissue distribution study.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2024-04-01 Epub Date: 2024-02-21 DOI:10.4155/bio-2023-0246

Lynn Tian, William Lafon, Qingguo Tian, Edward Tamer

引用次数: 0

Abstract

Aims: Cyanoenone triterpenoids penetrate the CNS, exhibiting biological activity via the nuclear factor E2-related factor (Nrf2) pathway. This is the first report on methods for the quantification of cyanonenone triterpenoids' distribution in various CNS tissues by LC-MS/MS. Materials & methods: The analyte was extracted from brain tissue homogenate using protein precipitation and supported liquid extraction. Results & conclusion: The assay validated a quantification range of 3.00-3000 ng/g in brain tissue samples as low as 5 mg. All parameters, including interference (≤20% at LLOQ) and accuracy/precision (15%, with 20% at LLOQ), met acceptance criteria. This assay supported a CNS distribution study, analyzing more than 10 mouse brain regions successfully.

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开发测定氰烯酮三萜类化合物的 LC-MS/MS 方法，以支持中枢神经系统组织分布研究。

目的：氰基烯酮三萜类化合物可穿透中枢神经系统，通过核因子 E2 相关因子（Nrf2）途径发挥生物活性。本文首次报道了利用 LC-MS/MS 对氰烯酮三萜类化合物在各种中枢神经系统组织中的分布进行定量的方法。材料与方法：采用蛋白沉淀和支撑液萃取法从脑组织匀浆中提取分析物。结果与结论：该检测方法在低至 5 毫克的脑组织样品中的定量范围为 3.00-3000 纳克/克。所有参数，包括干扰（LLOQ ≤20%）和准确度/精密度（15%，LLOQ 为 20%），均符合验收标准。该测定支持中枢神经系统分布研究，成功分析了 10 多个小鼠脑区。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.