Development of LC-MS/MS method for cyanoenone triterpenoid determination to support CNS tissue distribution study.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-01 Epub Date: 2024-02-21 DOI:10.4155/bio-2023-0246

Lynn Tian, William Lafon, Qingguo Tian, Edward Tamer

引用次数: 0

Abstract

Aims: Cyanoenone triterpenoids penetrate the CNS, exhibiting biological activity via the nuclear factor E2-related factor (Nrf2) pathway. This is the first report on methods for the quantification of cyanonenone triterpenoids' distribution in various CNS tissues by LC-MS/MS. Materials & methods: The analyte was extracted from brain tissue homogenate using protein precipitation and supported liquid extraction. Results & conclusion: The assay validated a quantification range of 3.00-3000 ng/g in brain tissue samples as low as 5 mg. All parameters, including interference (≤20% at LLOQ) and accuracy/precision (15%, with 20% at LLOQ), met acceptance criteria. This assay supported a CNS distribution study, analyzing more than 10 mouse brain regions successfully.

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开发测定氰烯酮三萜类化合物的 LC-MS/MS 方法，以支持中枢神经系统组织分布研究。

目的：氰基烯酮三萜类化合物可穿透中枢神经系统，通过核因子 E2 相关因子（Nrf2）途径发挥生物活性。本文首次报道了利用 LC-MS/MS 对氰烯酮三萜类化合物在各种中枢神经系统组织中的分布进行定量的方法。材料与方法：采用蛋白沉淀和支撑液萃取法从脑组织匀浆中提取分析物。结果与结论：该检测方法在低至 5 毫克的脑组织样品中的定量范围为 3.00-3000 纳克/克。所有参数，包括干扰（LLOQ ≤20%）和准确度/精密度（15%，LLOQ 为 20%），均符合验收标准。该测定支持中枢神经系统分布研究，成功分析了 10 多个小鼠脑区。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.