Establishing reference intervals for soluble urokinase plasminogen activator receptor in Northern European adults

IF 1.7 Q3 MEDICAL LABORATORY TECHNOLOGY

Practical Laboratory Medicine Pub Date : 2024-02-16 DOI:10.1016/j.plabm.2024.e00371

Stine Bakkensen Bruun , Jeppe Buur Madsen , Claus Lohman Brasen

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引用次数: 0

Abstract

Objectives

Soluble urokinase plasminogen activator receptor (suPAR) may have untapped potential in clinical diagnostics. Previous studies determined reference intervals using an enzyme-linked immunoassay, but there is a need for reference intervals using a faster assay if the analysis is to be used in emergency medicine. The current study aims to determine reference intervals for suPAR using a fully automated particle-enhanced turbidimetric immunoassay (PETIA) according to the Clinical and Laboratory Standards Institute guideline A28-A3c.

Design and methods

Blood samples were prospectively collected from Danish blood donors. Plasma suPAR was analyzed on the cobas 8000 module c502 in an open channel using a PETIA. Sex-partitioned reference intervals were determined using a parametric quantile approach.

Results

The study included 241 participants—123 females and 118 males. The common reference interval for suPAR was 1.56–4.11 ng/mL (95% confidence intervals (CI) for the lower and upper limits were 1.56–1.63 and 3.81–4.47, respectively). The reference interval for females was 1.59–4.65 ng/mL (95% CIs 1.48–1.70 and 4.09–5.48, respectively) and for males, 1.56–3.59 ng/mL (95% CIs 1.47–1.65 and 3.31–3.93, respectively).

Conclusions

Our results support using sex-partitioned reference intervals for suPAR and provide a basis for future studies using the PETIA method.

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确定北欧成年人可溶性尿激酶纤溶酶原激活物受体的参考区间

目的可溶性尿激酶纤溶酶原激活物受体（suPAR）在临床诊断中可能具有尚未开发的潜力。以前的研究使用酶联免疫测定法确定了参考区间，但如果要在急诊医学中使用该分析方法，则需要使用更快的测定方法确定参考区间。目前的研究旨在根据临床和实验室标准协会指南 A28-A3c，使用全自动粒子增强比浊免疫分析法（PETIA）确定 suPAR 的参考区间。血浆 suPAR 由 cobas 8000 模块 c502 在开放通道中使用 PETIA 进行分析。采用参数量化方法确定了按性别划分的参考区间。结果该研究包括 241 名参与者--123 名女性和 118 名男性。suPAR的共同参考区间为1.56-4.11纳克/毫升（下限和上限的95%置信区间（CI）分别为1.56-1.63和3.81-4.47）。女性的参考区间为 1.59-4.65 纳克/毫升（95% 置信区间分别为 1.48-1.70 和 4.09-5.48），男性的参考区间为 1.56-3.59 纳克/毫升（95% 置信区间分别为 1.47-1.65 和 3.31-3.93）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Practical Laboratory Medicine Health Professions-Radiological and Ultrasound Technology

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

7 weeks

期刊介绍： Practical Laboratory Medicine is a high-quality, peer-reviewed, international open-access journal publishing original research, new methods and critical evaluations, case reports and short papers in the fields of clinical chemistry and laboratory medicine. The objective of the journal is to provide practical information of immediate relevance to workers in clinical laboratories. The primary scope of the journal covers clinical chemistry, hematology, molecular biology and genetics relevant to laboratory medicine, microbiology, immunology, therapeutic drug monitoring and toxicology, laboratory management and informatics. We welcome papers which describe critical evaluations of biomarkers and their role in the diagnosis and treatment of clinically significant disease, validation of commercial and in-house IVD methods, method comparisons, interference reports, the development of new reagents and reference materials, reference range studies and regulatory compliance reports. Manuscripts describing the development of new methods applicable to laboratory medicine (including point-of-care testing) are particularly encouraged, even if preliminary or small scale.