Abortion associated with postpartum opportunistic bacterial invasion reduces fertility and induces disturbances of reproductive hormones, hematological profile, and oxidant/antioxidant profiles in dairy cows.
Yahia A Amin, Gamal A M Omran, Samer S Fouad, Mariam A Fawy, Rawia M Ibrahim, Fatma Ahmed Khalifa, Rana A Ali
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引用次数: 0
Abstract
Objective: The following study examines for the first time the changes that occur in the post-partum period following abortion in the first trimester of dairy cows using hormonal, hematological, and oxidant/antioxidant profiles. In addition, a bacteriological examination was also performed to explore the role of infections in the complications that occur during this period.
Materials and methods: One hundred cows were split into two equal groups: The first group enrolled cows that suffered from abortion in the first trimester. The second group enrolled cows that did not experience abortion problems (the control group). Uterine swabs were collected from cows. Blood samples were collected for hormonal, hematological, and oxidative profiles.
Results: Results reveal that Escherichia coli, Staphylococcus spp., and Streptococcus spp. are the opportunistic bacteria that were isolated from abortive cows with multidrug-resistant (MDR) characteristics. Red blood cell (RBC) count, hemoglobin, mean corpuscular hemoglobin (MCH), and MCH concentration (MCHC) were significantly higher in the abortive group than in controls in the first 3 days after calving. Conversely, total leukocyte count, platelet count, neutrophils, eosinophils, and immunoglobulin G and M were significantly lower in the abortion group than in controls. The concentrations of estradiol, prostaglandin F2α, oxytocin, and cortisol are significantly increased in the abortive cows, while progesterone is significantly decreased. The levels of malondialdehyde (MDA) were higher in the abortive group, while the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and total antioxidant capacity (TAC) were lower.
Conclusion: Abortion during the first trimester of pregnancy increases the risk of postpartum opportunistic bacterial invasion of the uterus. Oxidative stress (OS) and neutropenia are the most important findings that may occur in the postpartum period after abortion and may be due to the abortion itself or its predisposition to opportunistic bacterial invasion of the uterus, which finally causes a fertility reduction.
研究目的以下研究首次利用荷尔蒙、血液学和氧化剂/抗氧化剂图谱对奶牛头三个月流产后产后期间发生的变化进行了研究。此外，还进行了细菌学检查，以探讨感染在这一时期发生的并发症中的作用：100 头奶牛被分成两个相同的组：第一组是在妊娠头三个月流产的奶牛。第二组为未出现流产问题的奶牛（对照组）。收集奶牛的子宫拭子。采集血液样本，用于激素、血液学和氧化谱分析：结果显示，大肠杆菌、葡萄球菌和链球菌是流产奶牛体内分离出的机会性细菌，具有耐多药（MDR）特性。在产犊后头三天，流产组的红细胞（RBC）计数、血红蛋白、平均血红蛋白（MCH）和血红蛋白浓度（MCHC）明显高于对照组。相反，流产组的白细胞总数、血小板计数、中性粒细胞、嗜酸性粒细胞、免疫球蛋白 G 和 M 明显低于对照组。流产奶牛体内雌二醇、前列腺素 F2α、催产素和皮质醇的浓度明显升高，而孕酮则明显降低。流产组的丙二醛（MDA）水平较高，而超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和总抗氧化能力（TAC）水平较低：结论：妊娠头三个月的人工流产会增加产后机会性细菌侵入子宫的风险。氧化应激（OS）和中性粒细胞减少症是流产后产后可能出现的最重要的结果，可能是由于流产本身或流产易导致机会性细菌侵入子宫，最终导致生育能力下降。
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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