Repetitive DNA Mapping in Five Genera of Tree Frogs (Amphibia: Anura) from the Atlantic Forest: New Highlights on Genomic Organization in Hylidae.

IF 1.7 4区生物学 Q4 CELL BIOLOGY

Cytogenetic and Genome Research Pub Date : 2023-01-01 Epub Date: 2024-02-16 DOI:10.1159/000537875

Késsia Leite Souza, Silvana Melo, Marco Antônio Peixoto, Natália Martins Travenzoli, Renato Neves Feio, Jorge Abdala Dergam

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引用次数: 0

Abstract

Introduction: The tribes Cophomantini, Scinaxini, and Dendropsophini are anurans that belong to Hylidae, with wide distribution in tropical and subtropical regions around the world. The taxonomy and systematics of this family remain in a state of ongoing revision. Previous cytogenetic analyses of genera Boana, Bokermannohyla, Ololygon, Scinax, and Dendropsophus described some karyotypic characters such as conventional staining, C-banding and NORs, and FISH with specific probes.

Methods: This study describes for the first time the karyotypes of four species: Bokermannohyla ibitipoca, Ololygon luizotavioi, Dendropsophus bipunctatus, and Dendropsophus ruschii. Furthermore, we map CA(15) and CAT(10) microsatellite sites for the aforementioned species and six more species from the same genera for insight into the chromosomal evolution within the subfamily Hyalinae.

Results: B. ibitipoca and O. luizotavioi had 2n = 24 and karyotypic formulas 18m + 4sm + 2st and 8m + 12sm + 4st, while D. bipunctatus and D. ruschii showed 2n = 30 and karyotypic formulas 12m + 12sm + 4st + 2t and 10m + 10sm + 6st + 4t, respectively. The diploid numbers and karyotypic formulas revealed here follow the previously reported trend for Hylidae, except B. ibitipoca has a particularity of eight metacentric chromosomes, more than what is commonly found in species of this genus. The microsatellites probes CA(15) and CAT(10) had markings accumulated in blocks in the centromeric, pericentromeric, and terminal regions that were more specific for some species, as well as markings scattered along the chromosomes. We present a comprehensive review table of current data on cytogenetics of these genera.

Conclusion: Our findings showed that the karyotypes of the hylids studied here majority fit the postulated conserved diploid number (2n = 24) and morphological chromosome patterns, while the mapping of the microsatellites enabled us to detect differences between species that share similar chromosomal morphologies.

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大西洋森林五属树蛙（两栖类：无尾目）的重复 DNA 图谱：Hylidae 基因组组织的新亮点。

简介蟾蜍科（Cophomantini）、蟾蜍亚科（Schinaxini）和蟾蜍亚科（Dendropsophini）属于无尾目动物，广泛分布于世界各地的热带和亚热带地区。该科的分类学和系统学仍在不断修正之中。之前对 Boana、Bokermannohyla、Ololygon、Schinax 和 Dendropsophus 等属的细胞遗传学分析描述了一些核型特征，如常规染色、C-带和 NORs，以及使用特定探针的 FISH：本研究首次描述了 Bokermannohyla ibitipoca、Ololygon luizotavioi、Dendropsophus bipunctatus 和 Dendropsophus ruschii 四个物种的核型。此外，我们还绘制了上述物种和另外六个同属物种的 CA(15) 和 CAT(10) 微卫星位点图，以深入了解绣线菊亚科内的染色体进化情况：Bok. ibitipoca和O. luizotavioi的染色体为2n= 24，核型分别为18m+4sm+2st和8m+12sm+4st；D. bipunctatus和D. ruschii的染色体为2n= 30，核型分别为12m+12sm+4st+2t和10m+10sm+6st+4t。除了 Bok. ibitipoca 有 8 条偏心染色体外，其他物种的二倍体数目和核型公式与之前报道的 Hylidae 的趋势一致。微卫星探针 CA(15) 和 CAT(10) 的标记在中心染色单体、近中心染色单体和末端区域积聚成块，对某些物种更具特异性，同时标记也散布在染色体上。我们提交了一份关于这些种属细胞遗传学现有数据的综合综述表：我们的研究结果表明，本文所研究的杓鹬属的核型符合推测的保守的二倍体数目和染色体形态模式，而这些微卫星的图谱使我们能够发现染色体形态相似的物种之间的差异。

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来源期刊

Cytogenetic and Genome Research 生物-细胞生物学

CiteScore

3.10

自引率

5.90%

发文量

审稿时长

1 months

期刊介绍： During the last decades, ''Cytogenetic and Genome Research'' has been the leading forum for original reports and reviews in human and animal cytogenetics, including molecular, clinical and comparative cytogenetics. In recent years, most of its papers have centered on genome research, including gene cloning and sequencing, gene mapping, gene regulation and expression, cancer genetics, comparative genetics, gene linkage and related areas. The journal also publishes key papers on chromosome aberrations in somatic, meiotic and malignant cells. Its scope has expanded to include studies on invertebrate and plant cytogenetics and genomics. Also featured are the vast majority of the reports of the International Workshops on Human Chromosome Mapping, the reports of international human and animal chromosome nomenclature committees, and proceedings of the American and European cytogenetic conferences and other events. In addition to regular issues, the journal has been publishing since 2002 a series of topical issues on a broad variety of themes from cytogenetic and genome research.