Boric Acid Affects the Expression of DNA Double-Strand Break Repair Factors in A549 Cells and A549 Cancer Stem Cells: An In Vitro Study.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biological Trace Element Research Pub Date : 2024-11-01 Epub Date: 2024-02-17 DOI:10.1007/s12011-024-04082-y

Tuğba Semerci Sevimli, Aynaz Ghorbani, Bahar Demir Cevizlidere, Burcugül Altuğ, Murat Sevimli

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Abstract

DNA double-strand break (DSB) repair genes interact with tumor stemness- and resistance-associated processes in cancer stem cells (CSCs). Therefore, targeting DNA DSB genes in cancer treatment is important for the CSC phenotype. Although the anti-cancer effect of boric acid (BA) has been studied, its effect on DNA DSB is unclear. Moreover, no studies investigate BA's effects on DNA DSB of lung cancer stem cells (LC-SCs). To fill the gap, we aimed to assess the effects of BA on A549 cancer stem cells. CSCs were isolated from human non-small cell lung cancer cells (A549) and characterized by flow cytometry. Different concentrations of BA (at doses ranging from 1 to 100 mM) were applied to cancer stem cells. Cytotoxic activities were determined using the cell viability assay (MTT assay) at 24 and 48 h. Expression levels of DNA DSB genes that BRCA1, BRCA2, RAD51, KU70/80, ATM, and XRCC4 were evaluated by RT-qPCR. Additionally, immunofluorescence staining analysis was exploited for caspase-3 and E-cadherin. ATM expression increased significantly (p < 0.001). No significant change was observed in the expression of other genes. Moreover, BA up-regulated caspase-3 and E-cadherin expression. Consequently, we can say that BA affects DNA DSB and the apoptotic abilities of LC-SCs.

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硼酸影响 A549 细胞和 A549 癌症干细胞中 DNA 双链断裂修复因子的表达：体外研究

DNA双链断裂（DSB）修复基因与肿瘤干细胞（CSCs）的肿瘤干性和抗药性相关过程相互作用。因此，在癌症治疗中靶向DNA DSB基因对CSC表型非常重要。虽然硼酸（BA）的抗癌作用已得到研究，但其对DNA DSB的影响尚不清楚。此外，还没有研究调查硼酸对肺癌干细胞（LC-SCs）DNA DSB的影响。为了填补这一空白，我们旨在评估 BA 对 A549 癌症干细胞的影响。我们从人类非小细胞肺癌细胞（A549）中分离出癌症干细胞，并用流式细胞术对其进行了鉴定。对癌症干细胞施加不同浓度的 BA（剂量从 1 到 100 mM 不等）。通过 RT-qPCR 评估了 BRCA1、BRCA2、RAD51、KU70/80、ATM 和 XRCC4 等 DNA DSB 基因的表达水平。此外，还对 Caspase-3 和 E-cadherin 进行了免疫荧光染色分析。ATM的表达明显增加（p

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来源期刊

Biological Trace Element Research 生物-内分泌学与代谢

CiteScore

8.70

自引率

10.30%

发文量

459

审稿时长

2 months

期刊介绍： Biological Trace Element Research provides a much-needed central forum for the emergent, interdisciplinary field of research on the biological, environmental, and biomedical roles of trace elements. Rather than confine itself to biochemistry, the journal emphasizes the integrative aspects of trace metal research in all appropriate fields, publishing human and animal nutritional studies devoted to the fundamental chemistry and biochemistry at issue as well as to the elucidation of the relevant aspects of preventive medicine, epidemiology, clinical chemistry, agriculture, endocrinology, animal science, pharmacology, microbiology, toxicology, virology, marine biology, sensory physiology, developmental biology, and related fields.