Human extracellular matrix (ECM)-like collagen and its bioactivity

IF 5.6 1区医学 Q1 MATERIALS SCIENCE, BIOMATERIALS

Regenerative Biomaterials Pub Date : 2024-02-01 DOI:10.1093/rb/rbae008

Hui Zhou, Wenwei Li, Lixin Pan, Tianci Zhu, Teng Zhou, E. Xiao, Qiang Wei

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Abstract

Collagen, the most abundant structural protein in the human extracellular matrix (ECM), provides essential support for tissues and guides tissue development. Despite its widespread use in tissue engineering, there remains uncertainty regarding the optimal selection of collagen sources. Animal-derived sources pose challenges such as immunogenicity, while the recombinant system is hindered by diminished bioactivity. In this study, we hypothesized that human ECM-like collagen (hCol) could offer an alternative for tissue engineering. In this study, a facile platform was provided for generating hCol derived from mesenchymal stem cells (MSCs) with a hierarchical structure and biochemical properties resembling native collagen. Our results further demonstrated that hCol could facilitate basal biological behaviors of human adipose-derived stem cells (hASCs), including viability, proliferation, migration and adipocyte-like phenotype. Additionally, it could promote cutaneous wound closure. Due its high similarity to native collagen and good bioactivity, hCol holds promise as a prospective candidate for in vitro and in vivo applications in tissue engineering.

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类细胞外基质（ECM）胶原蛋白及其生物活性

胶原蛋白是人体细胞外基质（ECM）中最丰富的结构蛋白，为组织提供重要的支撑并引导组织发育。尽管胶原蛋白广泛应用于组织工程，但在胶原蛋白来源的最佳选择方面仍存在不确定性。动物来源会带来免疫原性等挑战，而重组系统则会因生物活性降低而受到阻碍。在本研究中，我们假设人 ECM 样胶原蛋白（hCol）可作为组织工程的替代品。在这项研究中，我们提供了一个简便的平台，用于生成由间充质干细胞（MSCs）衍生的 hCol，它具有分层结构和类似于原生胶原蛋白的生化特性。我们的研究结果进一步证明，hCol 可促进人脂肪干细胞（hASCs）的基本生物学行为，包括活力、增殖、迁移和类脂肪细胞表型。此外，它还能促进皮肤伤口闭合。由于 hCol 与本地胶原蛋白高度相似且具有良好的生物活性，因此有望成为组织工程中体外和体内应用的候选材料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regenerative Biomaterials Materials Science-Biomaterials

CiteScore

7.90

自引率

16.40%

发文量

审稿时长

10 weeks

期刊介绍： Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.