3D bioprinting strategies for recapitulation of hepatic structure and function in bioengineered liver: A state-of-the-art review

IF 4.7 3区 工程技术 Q2 ENGINEERING, BIOMEDICAL
Arka Sanyal, Sourabh Ghosh
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Abstract

3D bioprinting has recently emerged as a successful biofabrication strategy for replicating the complex in vivo hepatic milieu. Significant research advances in this field have allowed for the fabrication of biomimetic hepatic tissues with potential applications in the healthcare (regeneration, transplantation, drug discovery) and diagnostic sectors (in vitro disease models). This article initially delves into describing the hepatic tissue architecture and function, followed by a rational exposition of how 3D bioprinting potentiates the better development of functional liver tissue compared to traditional tissue engineering approaches and 3D cell culture platforms. This review then highlights the recent breakthroughs and reliable strategies for replicating liver structure and function through bioprinting approaches. In this context, we have systematically described the current landscape of hepatic bioprinting, initially focusing on the cell sources used, followed by the biomaterials and strategies implemented to prolong their in vitro viability. Proceeding forward, we have critically highlighted essential aspects of hepatic bioprinting, such as developing tissue-specific bioinks, strategies to induce vascularization within bioprinted liver constructs, and replication of native liver tissue heterogeneity through spatial distribution of multiple cell types in predetermined patterns. In our concluding remarks, we discuss the existing bottlenecks that prevail in this field and provide our viewpoint regarding possible future directions to overcome them.

Abstract Image

在生物工程肝脏中再现肝脏结构和功能的三维生物打印策略:最新进展综述
最近,三维生物打印已成为一种成功的生物制造策略,可用于复制复杂的体内肝脏环境。该领域的重大研究进展使得生物仿真肝组织的制造成为可能,可应用于医疗保健(再生、移植、药物发现)和诊断领域(体外疾病模型)。本文首先深入探讨了肝组织的结构和功能,然后理性阐述了与传统的组织工程方法和三维细胞培养平台相比,三维生物打印如何促进功能性肝组织的更好发展。随后,本综述重点介绍了通过生物打印方法复制肝脏结构和功能的最新突破和可靠策略。在此背景下,我们系统地描述了肝脏生物打印的现状,首先重点介绍了所使用的细胞来源,然后介绍了生物材料和延长体外存活率的策略。接着,我们批判性地强调了肝脏生物打印的重要方面,如开发组织特异性生物墨水、在生物打印肝脏构建体中诱导血管化的策略,以及通过多种细胞类型在预定模式中的空间分布复制原生肝脏组织的异质性。在结束语中,我们讨论了该领域目前存在的瓶颈,并就克服这些瓶颈的未来可能方向提出了自己的观点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Opinion in Biomedical Engineering
Current Opinion in Biomedical Engineering Medicine-Medicine (miscellaneous)
CiteScore
8.60
自引率
2.60%
发文量
59
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