Dietary violacein suppresses the deleterious effects of experimentally induced coccidiosis in broiler chickens

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Applied Poultry Research Pub Date : 2024-02-11 DOI:10.1016/j.japr.2024.100416

Shan R. Nawarathne , Hyesuk Kim , Myunghwan Yu , Hyun-Min Cho , Jun-Seon Hong , Yubin Kim , Samiru S. Wickramasuriya , Sungtaek Oh , Kyu-Yeol Son , Min-Ah Park , Kyungmin Lee , Hwi-Jae Kim , Young-Joo Yi , Jung-Min Heo

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引用次数: 0

Abstract

Poultry coccidiosis is a catastrophic parasitic disease and the industry is currently seeking alternative compounds to replace conventional antibiotics. Accordingly, the current study determined the effect of dietary violacein supplementation on growth performance and anticoccidial indices of Eimeria-infected broiler chickens. A total of 336-day-old “Ross 308” broiler chicks were allotted to 6 treatments: that is, positive control (PC), negative control (NC), 1 treatment with salinomycin (6 ppm), and 3 treatments with different violacein concentrations (10, 15, 20 ppm). Experimental coccidiosis was induced in all chickens (except NC) by inoculating 3.2 × 10⁵ sporulated oocysts. The treatment with 15 ppm violacein (VIO 15) significantly improved chicken growth performance compared to PC. Furthermore, significant reductions in lesion score, oocyst per gram of feces (OPG), and oocysts index (OI) were observed in the VIO 15 and the salinomycin treatments compared to other treatments. Violacein was reported as ‘moderately effective’ on Eimeria spp. based on the “anticoccidial index (ACI),” whereas “reduction in lesion score (RLS)” and “percentage anticoccidial activity (POAA)” indices designated its 15 ppm treatment as “highly sensitive” on them. Additionally, VIO 15 downregulated “interleukin-8 (IL-8)” and “interleukin-6 (IL-6)” compared to PC, in both spleen and cecal tonsils. Besides, expression levels of “chemokine C–C motif ligand 2 (CCLi-2),” were reduced in cecal tonsils of VIO 15 broiler chickens compared to PC. Conclusively, dietary violacein supplementation suppressed the deleterious effects of coccidial infection in broiler chickens with improved growth, and its 15 ppm level is recommended for use.

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膳食中的黄连素可抑制实验性球虫病对肉鸡的有害影响

家禽球虫病是一种灾难性寄生虫病，目前该行业正在寻找替代化合物来取代传统抗生素。因此，本研究确定了日粮中添加紫草素对感染艾美耳病的肉鸡生长性能和抗球虫指数的影响。将 336 日龄的 "Ross 308 "肉用仔鸡分为 6 个处理：即阳性对照（PC）、阴性对照（NC）、1 个使用盐霉素（6 ppm）的处理和 3 个使用不同浓度（10、15、20 ppm）的紫草素的处理。给所有鸡（NC 除外）接种 3.2 × 105 个孢子化卵囊，诱发实验性球虫病。与 PC 相比，使用 15 ppm 的紫草素（VIO 15）可显著改善鸡的生长性能。此外，与其他处理相比，VIO 15 和盐霉素处理的病变评分、每克粪便卵囊数（OPG）和卵囊指数（OI）均有明显降低。根据 "抗球虫指数（ACI）"，Violacein 对艾美耳菌属 "中等有效"，而 "病变评分减少（RLS）"和 "抗球虫活性百分比（POAA）"指数则表明，VIO 15 ppm 处理对它们 "高度敏感"。此外，与 PC 相比，VIO 15 还能降低脾脏和盲肠扁桃体中 "白细胞介素-8（IL-8）"和 "白细胞介素-6（IL-6）"的表达水平。此外，与 PC 相比，VIO 15 肉鸡盲肠扁桃体中 "趋化因子 C-C motif ligand 2 (CCLi-2) "的表达水平也有所降低。总之，日粮中添加紫草素能抑制球虫感染对肉鸡的有害影响并改善生长，建议使用 15 ppm 水平的紫草素。

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来源期刊

Journal of Applied Poultry Research 农林科学-奶制品与动物科学

CiteScore

4.10

自引率

10.50%

发文量

审稿时长

104 days

期刊介绍： The Journal of Applied Poultry Research (JAPR) publishes original research reports, field reports, and reviews on breeding, hatching, health and disease, layer management, meat bird processing and products, meat bird management, microbiology, food safety, nutrition, environment, sanitation, welfare, and economics. As of January 2020, JAPR will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. The readers of JAPR are in education, extension, industry, and government, including research, teaching, administration, veterinary medicine, management, production, quality assurance, product development, and technical services. Nutritionists, breeder flock supervisors, production managers, microbiologists, laboratory personnel, food safety and sanitation managers, poultry processing managers, feed manufacturers, and egg producers use JAPR to keep up with current applied poultry research.