Diarylheptanoid 7-(3,4-Dihydroxyphenyl)-5- Hydroxy-1-Phenyl- (1E)-1-Heptene from Curcuma Comosa Roxb. Inhibits Nucleophosmin Localization and Induces Apoptosis in KG-1a Leukemic Stem Cells

Journal of Health Science and Medical Research Pub Date : 2024-02-06 DOI:10.31584/jhsmr.20241034

Natsima Viriyaadhammaa, S. Duangmano, Pawaret Panyajai, K. Gyi, Singkome Tima, S. Chiampanichayakul, S. Okonogi, Pornngarm Dejkriengkraikul, Jeffrey Krise, A. Saiai, S. Anuchapreeda

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Abstract

Objective: This study aimed to investigate the effect of the diarylheptanoid 7-(3,4-dihydroxyphenyl)-5- hydroxy-1-phenyl-(1E)-1-heptene (DDHPH) from the rhizome of Curcuma comosa Roxb. on nucleophosmin (NPM) protein localization followed by induced cell death in KG-1a leukemic stem cells. Material and Methods: DDHPH was purified by column chromatography and characterized by nuclear magnetic resonance (NMR). Localization of NPM was determined by a confocal microscopy. Induction of cell death was determined by flow cytometry and Western blotting. Results: A previous study showed that NPM directly interacted with p53 and activates p53 function. In this study, DDHPH was found to modify the translocation of NPM and enhance both p53 and cleaved caspase-3 expression. These proteins were found to control cell cycle progression and cell apoptosis induction. Conclusion: This study demonstrated that DDHPH is a novel compound which induces cell death through NPM localization.

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莪术中的 7-(3,4-二羟基苯基)-5-羟基-1-苯基-(1E)-1-庚烯二酮抑制核苷酸定位并诱导 KG-1a 白血病干细胞凋亡

研究目的本研究旨在探讨莪术根茎中的二芳基庚烷类化合物7-(3,4-二羟基苯基)-5-羟基-1-苯基-(1E)-1-庚烯(DDHPH)对KG-1a白血病干细胞中的核糖蛋白(NPM)定位及诱导细胞死亡的影响。材料与方法用柱层析法纯化 DDHPH，并用核磁共振（NMR）对其进行表征。共聚焦显微镜确定了 NPM 的定位。通过流式细胞术和 Western 印迹法测定细胞死亡诱导。结果：之前的一项研究表明，NPM 直接与 p53 相互作用并激活 p53 的功能。本研究发现，DDHPH 可改变 NPM 的转位，并增强 p53 和裂解的 Caspase-3 的表达。研究发现，这些蛋白可控制细胞周期的进展和诱导细胞凋亡。结论本研究表明，DDHPH 是一种新型化合物，可通过 NPM 定位诱导细胞死亡。

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Journal of Health Science and Medical Research

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