The Effect of Varied Time Interval and Micro-Current (Direct) on the Level of Biomarker (IL-6) and Rate of Tooth Movement: An Animal Study

Q3 Dentistry
Parnita Dwivedi, Ashish Agrawal, Subash Chandra Gupta, Tehmin Chou
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引用次数: 0

Abstract

Exogenous application of direct current (DC) on piezoelectric biopolymers results in biochemical modifications in the intracellular/extracellular regions which profoundly affects the pace of bone turnover. A qualitative examination of DC (waveform and frequency) provides ideal qualities of current. 20 female Wistar rats (Rattus norvegicus) were randomly selected and divided into control (group 1–4 rats) with orthodontic appliance (OA) and experimental groups (OA + Micro-current (MC). Experimental groups were subdivided into four groups (group 2–20 μA/5 sec, 3-20 μA/10 sec, 4-15 μA/5 sec, 5-15 μA/10 sec with four rats in each). The tooth movement was recorded every 24th hours for 7 days. Gingival crevicular fluid (GCF) was collected 6 hr, 12 hr, 24 hr, 3rd day and 168 hrs with the absorbent paper points at specific location around the tooth in control and experiment groups. Histopathological analysis was done on 168 hrs to assess the osteoclastic activity around the root. Interleukin-6 (IL-6) concentration was accessed using enzyme-linked immunosorbent assay (ELISA). The data were subjected to one-way ANOVA and Tukey’s post hoc test. There was a statistically significant difference in tooth movement, group 3 (20 μA/10 sec) showed an increased rate of tooth movement on the 168 hrs. The level of IL-6 was maximum on 72 hrs in experimental group 3. The study showed time interval/frequency, the waveform, and the amount of current play a significant role in determining bone turnover and rate of tooth movement.
不同时间间隔和微电流(直流)对生物标志物(IL-6)水平和牙齿移动速度的影响:一项动物研究
在压电生物聚合物上施加外源性直流电(DC)会导致细胞内/外区域的生化变化,从而对骨转换速度产生深远影响。直流电的定性检查(波形和频率)提供了理想的电流质量。 随机选取 20 只雌性 Wistar 大鼠(Rattus norvegicus),将其分为使用正畸器(OA)的对照组(1-4 组)和实验组(OA + 微电流(MC))。实验组又分为四组(2-20 μA/5秒组、3-20 μA/10秒组、4-15 μA/5秒组、5-15 μA/10秒组,每组四只大鼠)。每 24 小时记录一次牙齿移动情况,持续 7 天。对照组和实验组分别于 6 小时、12 小时、24 小时、第 3 天和 168 小时在牙齿周围的特定位置用吸水纸点收集牙龈缝液(GCF)。168 小时后进行组织病理学分析,以评估牙根周围的破骨活性。使用酶联免疫吸附试验(ELISA)检测白细胞介素-6(IL-6)的浓度。对数据进行单因素方差分析和 Tukey 后检验。 牙齿移动在统计学上有显著差异,第 3 组(20 μA/10 秒)的牙齿移动速度在 168 小时内有所增加。研究表明,时间间隔/频率、波形和电流大小在决定骨转换和牙齿移动速度方面起着重要作用。
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来源期刊
Indian Journal of Dental Research
Indian Journal of Dental Research Dentistry-Dentistry (all)
CiteScore
1.80
自引率
0.00%
发文量
80
审稿时长
38 weeks
期刊介绍: Indian Journal of Dental Research (IJDR) is the official publication of the Indian Society for Dental Research (ISDR), India section of the International Association for Dental Research (IADR), published quarterly. IJDR publishes scientific papers on well designed and controlled original research involving orodental sciences. Papers may also include reports on unusual and interesting case presentations and invited review papers on significant topics.
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