Diagnostic precision of Truenat® technique and co-relation of ALT levels with HBV-DNA viral load among HBsAg positive patients at a tertiary care hospital in Eastern Uttar Pradesh

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2024-02-12 DOI:10.18502/ijm.v16i1.14882

Sarita Kumari, Bechan Kumar Gautam, A. Singh, Vivek Gaur, Ankur Kumar

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引用次数: 0

Abstract

Background and Objectives: In India, it is estimated that there are 40 million people suffering from Hepatitis B virus (HBV). Quantification of the viral burden is an important laboratory tool in the management. However, widespread use of different HBV-DNA assays is still affected by the high cost and variable diagnostic precision. The present study was conduct- ed to evaluate the diagnostic precision and co-relation of ALT levels with HBV-DNA by Truenat®-PCR. Materials and Methods: In this prospective cross-sectional study a total of 567 serums were collected from patients by rapid HBsAg, and processed for liver function tests (LFT). The viral HBV-DNA amplification detection was carried out through by Truenat®-PCR test. Results: Out of 567 samples, 452 samples were found to be positive by both rapid and Truenat®-PCR and 106 were negative for HBV-DNA followed by 9 invalid. High ALT level found in 73% of positive patients who had HBV-DNA level (>100000 copies/ml) which is significantly higher in 447 patients as compared to those have below ≤100000 copies/ml. Conclusion: Truenat®-PCR technique is a highly sensitive and can be performed with low resources for effective control of HBV infection. Evaluation of HBV-DNA levels and serum ALT levels showed a significant proportion of patient harbored ongoing viral replication and disease progression.

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Truenat® 技术的诊断精度以及北方邦东部一家三级医院中 HBsAg 阳性患者的 ALT 水平与 HBV-DNA 病毒载量的相关性

背景与目标：据估计，印度有 4000 万乙型肝炎病毒（HBV）感染者。病毒负担的量化是管理中的一个重要实验室工具。然而，各种 HBV DNA 检测方法的广泛使用仍受到高成本和诊断精确度不一的影响。本研究旨在通过 Truenat®-PCR 评估 ALT 水平与 HBV-DNA 的诊断精确度和相关性。材料和方法：在这项前瞻性横断面研究中，通过快速 HBsAg 采集了患者的 567 份血清，并进行了肝功能检测（LFT）。病毒 HBV-DNA 扩增检测通过 Truenat®-PCR 测试进行。结果显示在 567 份样本中，452 份样本经快速和 Truenat®-PCR 检测均呈阳性，106 份样本 HBV-DNA 阴性，9 份样本无效。在 HBV-DNA 水平（>100000 拷贝/毫升）呈阳性的患者中，有 73% 发现 ALT 水平较高，其中 447 例患者的 ALT 水平明显高于低于 100000 拷贝/毫升的患者。结论Truenat®-PCR 技术灵敏度高，只需少量资源就能有效控制 HBV 感染。对 HBV-DNA 水平和血清 ALT 水平的评估显示，相当一部分患者的病毒复制仍在进行，疾病仍在恶化。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.