Xue Li, Xiao Xiao, Lei Wang, Weichun Liang, Jun Ruan, Jianyi Ou
{"title":"A Sustained-Release SP-Sr-CaS/NBD Microsphere for Promoting Bone Repair and Inhibiting Inflammation for the Treatment of Osteomyelitis","authors":"Xue Li, Xiao Xiao, Lei Wang, Weichun Liang, Jun Ruan, Jianyi Ou","doi":"10.1166/jbn.2024.3762","DOIUrl":null,"url":null,"abstract":"In this study, we prepare sustained-release poly(lactide-co-glycolide) (PLGA)-based microspheres (SP), containing strontium (Sr), calcium sulfate (CaS), and NF-κB essential modifier-binding domain (NBD) peptide, namely SP-Sr-CaS/NBD, for the treatment of osteomyelitis.\n Our results demonstrate that the SP-Sr-CaS/NBD group exhibited enhanced bone repair speed and infection clearance rate compared to other groups. Moreover, histological staining revealed more comprehensive bone structure restoration in the SP-Sr-CaS/NBD group. Furthermore, we assessed the levels\n of bone growth factors and apoptosis factors in primary bone marrow mesenchymal stem cells and found that NBD effectively suppresses inflammation while Sr-CaS promotes bone healing by inhibiting cell apoptosis. Additionally, we conducted in vitro and in vivo toxicity evaluations\n of the microspheres, which confirmed their potential as a competitive filling material for osteomyelitis. In conclusion, SP-Sr-CaS/NBD microspheres hold great promise as therapeutic scaffolds for clinical cases involving bone infections by reducing pain and treatment duration. This study provides\n a new repair material for the treatment of osteomyelitis and promotes the development of repair material for osteomyelitis.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical nanotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbn.2024.3762","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, we prepare sustained-release poly(lactide-co-glycolide) (PLGA)-based microspheres (SP), containing strontium (Sr), calcium sulfate (CaS), and NF-κB essential modifier-binding domain (NBD) peptide, namely SP-Sr-CaS/NBD, for the treatment of osteomyelitis.
Our results demonstrate that the SP-Sr-CaS/NBD group exhibited enhanced bone repair speed and infection clearance rate compared to other groups. Moreover, histological staining revealed more comprehensive bone structure restoration in the SP-Sr-CaS/NBD group. Furthermore, we assessed the levels
of bone growth factors and apoptosis factors in primary bone marrow mesenchymal stem cells and found that NBD effectively suppresses inflammation while Sr-CaS promotes bone healing by inhibiting cell apoptosis. Additionally, we conducted in vitro and in vivo toxicity evaluations
of the microspheres, which confirmed their potential as a competitive filling material for osteomyelitis. In conclusion, SP-Sr-CaS/NBD microspheres hold great promise as therapeutic scaffolds for clinical cases involving bone infections by reducing pain and treatment duration. This study provides
a new repair material for the treatment of osteomyelitis and promotes the development of repair material for osteomyelitis.