Senyawa Bioaktif Kappaphycus alvarezii (Doty) Doty, 1988 Sebagai Antibakteri

IF 0.5 4区地球科学 Q3 Earth and Planetary Sciences

Journal of Marine Research Pub Date : 2024-02-03 DOI:10.14710/jmr.v13i1.40459

Annisa Marhaeny, Rini Pramesti, S. Suryono

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引用次数: 0

Abstract

Bakteri yang menyebabkan penyakit pada inangnya disebut patogen. Penelitian ini bertujuan mengetahui senyawa bioaktif dan aktivitas antibakteri yang terkandung dalam ekstrak metanol K. alvarezii. Penelitian dilakukan dengan metode deskriptif eksploratif. Bakteri yang diujikan yaitu Staphylococcus aureus dan Escherichia coli. Hasil ekstraksi dilanjutkan dengan uji fitokimia. Ekstrak diencerkan menggunakan dimetil sulfoksida (DMSO) sampai diperoleh konsentrasi uji 500 µg/disk, 1000 µg/disk, dan 1500 µg/disk. Cefadroxil dan DMSO digunakan sebagai kontrol positif dan kontrol negatif. Metode difusi cakram digunakan untuk uji aktivitas antibakteri. Inokulum diinkubasi selama 3x24 jam pada suhu 37ºC. Zona hambat dihitung setiap 24 jam sekali. Hasil uji aktivitas antibakteri ekstrak metanol K. alvarezii terhadap bakteri S. aureus dan E. coli menunjukkan zona hambat terbesar pada konsentrasi 500 µg/disk pada waktu 48 jam sebesar 4,95 mm dan 5,1 mm. Senyawa bioaktif yang terkandung dalam ekstrak metanol K. alvarezii yaitu senyawa alkaloid, flavonoid, steroid dan tanin. Bacteria that cause disease in their hosts are called pathogens. This study aims to determine the bioactive compounds and antibacterial activity in the methanol extract of K. alvarezii. The research was conducted using a exploratory descriptive method. The bacteria tested were Staphylococcus aureus and Escherichia coli. Extraction then phytochemical tests were carried out The extract was diluted using dimethyl sulfoxide (DMSO) to obtain test concentrations of 500 µg/disk, 1000 µg/disk, and 1500 µg/disk. Cefadroxil and DMSO were used as positive and negative controls. Antibacterial activity test using disc diffusion method. The inoculum was incubated for 3x24 hours at 37°C. The inhibition zone is calculated every 24 hours. The results of the antibacterial activity test of K. alvarezii methanol extract against S. aureus and E. coli bacteria showed the largest inhibition zone at a concentration of 500 µg/disk in 48 hours of 4.95 mm and 5.1 mm. The bioactive compounds contained in the methanol extract of K. alvarezii are alkaloids, flavonoids, steroids and tannins.

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1988 年 Kappaphycus alvarezii (Doty) Doty 作为抗菌剂的生物活性化合物

在农村地区，酵母菌会引起疾病。该研究的目的是在甲醇 K. alvarezii 的代谢过程中获得生物活性和抗酵母菌活性。该研究采用了一种可视化的方法。金黄色葡萄球菌和大肠埃希氏菌都是被感染的细菌。这些细菌都是通过酵母菌培养出来的。试验中使用的二甲基亚砜（DMSO）浓度分别为 500 微克/盘、1000 微克/盘和 1500 微克/盘。头孢羟氨苄和二甲基亚砜分别作为阳性对照和阴性对照。抗菌肽检测方法用于提高抗菌肽的活性。在 37ºC 温度下浸泡 3x24 小时。在一天中的 24 小时内完成对疣体的处理。金黄色葡萄球菌和大肠埃希氏菌的抗菌剂 K. alvarezii 的活性在 48 小时内按 500 µg/disk 的浓度增加到 4.95 mm 和 5.1 mm。阿尔瓦雷茨甲醇中的生物碱、类黄酮、类固醇和鞣质。对宿主造成疾病的细菌被称为病原体。本研究旨在确定 K. alvarezii 甲醇提取物中的生物活性化合物和抗菌活性。研究采用探索性描述方法进行。测试的细菌为金黄色葡萄球菌和大肠杆菌。提取物用二甲基亚砜（DMSO）稀释，以获得 500 微克/盘、1000 微克/盘和 1500 微克/盘的测试浓度。头孢羟氨苄和二甲基亚砜分别作为阳性和阴性对照。采用盘扩散法进行抗菌活性测试。接种物在 37°C 下培养 3x24 小时。每 24 小时计算抑菌区。K. alvarezii 甲醇提取物对金黄色葡萄球菌和大肠杆菌的抗菌活性测试结果表明，浓度为 500 µg/disk 时，48 小时内最大抑菌区分别为 4.95 毫米和 5.1 毫米。K. alvarezii甲醇提取物中含有的生物活性化合物有生物碱、黄酮类、类固醇和单宁酸。

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来源期刊

Journal of Marine Research 地学-海洋学

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： The Journal of Marine Research publishes peer-reviewed research articles covering a broad array of topics in physical, biological and chemical oceanography. Articles that deal with processes, as well as those that report significant observations, are welcome. In the area of biology, studies involving coupling between ecological and physical processes are preferred over those that report systematics. Authors benefit from thorough reviews of their manuscripts, where an attempt is made to maximize clarity. The time between submission and publication is kept to a minimum; there is no page charge.