Single nucleotide polymorphism (SNP) marker-assisted breeding of Heterorhabditis bacteriophora for improvement of reproductive potential and stress tolerance

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-02-06 DOI:10.1163/15685411-bja10307

Christopher Ogaya, Michelle Ann B. Diano, Innocent Hategekimana, Verena Dörfler, Carlos Molina, Ralf-Udo Ehlers

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Abstract

The entomopathogenic nematode (EPN), Heterorhabditis bacteriophora, is an efficient biological control agent against several economically important insect pests. Recent research has assessed the possibility to correlate desired beneficial traits with genotype data to pave a way for marker-assisted breeding approaches. A collection of H. bacteriophora WT inbred lines has been phenotyped in this framework for stress- and virulence-related traits. However, these traits are rarely combined in a single line. Thus, unifying these traits in commercial strains is of high priority. This investigation unified beneficial traits in hybrid pools through marker-assisted breeding using single nucleotide polymorphisms (SNPs) associated with reproductive potential, longevity, virulence and cold tolerance. Recombinant inbred lines (RILs) generated from a cross between a stress tolerant WT inbred line (XX21) and a line high in in vitro reproduction potential (IL3) were genotyped via SeqSNP and screened for SNP markers associated with beneficial traits. Thereafter, a genotypic pool (X21L3) comprising 22 ILs was formed. The X21L3 pool was subsequently evaluated for the target traits in comparison with the cross parents and a commercial strain HB4. An improvement of oxidative stress tolerance at 2°C (cold tolerance) was recorded with X21L3 surviving 1 day longer than the best performing parent (XX21). The hybrid pool also survived 1 day longer than the least performing parent IL3 for the trait longevity at 25°C under oxidative stress conditions. A higher dauer juvenile (DJ) recovery (58%) and DJ yield (209 000 DJ ml−1) than the least performing parent XX21 was recorded for the pool. The storage stability in diatomaceous earth formulation at 2°C and 7.5°C was also improved by 2 and 5 days, respectively, in comparison to the least performing parent XX21. This study depicts the potential of precision marker-assisted breeding for beneficial trait improvement of H. bacteriophora.

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单核苷酸多态性（SNP）标记辅助细菌杂环虫育种以提高繁殖潜力和抗逆性

昆虫病原线虫（EPN）--细菌异habditis bacteriophora--是一种高效的生物防治剂，可防治多种具有重要经济价值的害虫。最近的研究评估了将所需的有益性状与基因型数据相关联的可能性，从而为标记辅助育种方法铺平道路。在这一框架下，已对一系列菌核病 WT 近交系进行了压力和毒力相关性状的表型分析。然而，这些性状很少结合在一个品系中。因此，在商业品系中统一这些性状是当务之急。这项研究通过使用与繁殖潜力、寿命、毒力和耐寒性相关的单核苷酸多态性（SNPs）进行标记辅助育种，统一了杂交种池中的有益性状。通过 SeqSNP 对抗逆性强的 WT 近交系（XX21）和体外繁殖潜力高的品系（IL3）杂交产生的重组近交系（RIL）进行基因分型，并筛选与有益性状相关的 SNP 标记。之后，形成了一个由 22 个 IL 组成的基因型库（X21L3）。随后，通过与杂交亲本和商业菌株 HB4 的比较，对 X21L3 基因库的目标性状进行了评估。结果表明，X21L3 比表现最好的亲本（XX21）多存活 1 天，从而提高了在 2°C 下的氧化应激耐受性（耐寒性）。在 25°C 的氧化胁迫条件下，杂种池的寿命也比表现最差的亲本 IL3 长 1 天。与表现最差的亲本 XX21 相比，该杂交种池的 Dauer 幼虫（DJ）恢复率（58%）和 DJ 产量（209 000 DJ ml-1）都更高。与性能最差的亲本 XX21 相比，硅藻土制剂在 2°C 和 7.5°C 下的储存稳定性也分别提高了 2 天和 5 天。这项研究展示了精确标记辅助育种在改良菌核病有益性状方面的潜力。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.