Early detection of oral bacteria causing gum infections and dental caries in children

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2024-02-10 DOI:10.18502/ijm.v16i1.14870

Maryam Darabi, Ronak Bakhtiari, Ahmad Jafari, Majid Mehran, Seyed Saeed Eshraghi, Anis Barati

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引用次数: 0

Abstract

Background and Objectives: Periodontal diseases are resulted from gum infections and dental plaques, which are mainly caused by the bacterial agents. Since dental monitoring includes important prognostic roles, the aim of this study was to detect the most common periodontal pathogenic bacteria in children. Materials and Methods: A total of 200 clinical samples were collected from dental plaques and gingival grooves. Tar- get-specific primers were designed for hbpA in Aggregatibacter actinomycetemcomitans, fimA in Porphyromonas gingivalis and 16S rRNA in Prevotella intermedia, Tannerella forsythia and Treponema denticola. Then, a multiplex polymerase chain reaction method was optimized for the highlighted bacterial agents. Results: In general, the highest and the lowest bacterial prevalence rates belonged to Tannerella forsythia (88%) and Por- phyromonas gingivalis (13%), respectively. Furthermore, prevalence rates of Aggregatibacter actinomycetemcomitans, Prevotella intermedia and Treponema denticola were 25, 21 and 45% in samples, respectively. Conclusion: There were significant associations between dental/oral health and microbial community. Metabolism of the oral bacteria, including biofilm formation, can affect gums and develop dental plaques and hence dental caries, especially in children. Early diagnosis of dental caries in children via rapid, accurate molecular methods can increase the diagnostic capacity in clinical cases and therefore prevent periodontal infections in adulthood.

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早期检测导致儿童牙龈感染和龋齿的口腔细菌

背景和目的：牙周疾病是由牙龈感染和牙菌斑引起的，而牙龈感染和牙菌斑主要是由细菌引起的。由于牙科监测具有重要的预后作用，因此本研究旨在检测儿童中最常见的牙周致病菌。材料和方法：从牙菌斑和龈沟共采集了 200 份临床样本。设计了针对放线菌（Aggregatibacter actinomycetemcomitans）的 hbpA 引物、牙龈卟啉单胞菌（Porphyromonas gingivalis）的 fimA 引物以及中间普雷沃特氏菌（Prevotella intermedia）、连翘坦奈氏菌（Tannerella forsythia）和牙髓震颤素（Treponema denticola）的 16S rRNA 引物。然后，优化了针对重点细菌病原体的多重聚合酶链反应方法。结果显示一般来说，细菌感染率最高和最低的分别是连翘丹那菌（88%）和牙龈卟啉单胞菌（13%）。此外，牙龈放线杆菌（Aggregatibacter actinomycetemcomitans）、中间前驱菌（Prevotella intermedia）和牙髓震颤素（Treponema denticola）的感染率分别为 25%、21% 和 45%。结论牙齿/口腔健康与微生物群落之间存在明显的关联。口腔细菌的新陈代谢，包括生物膜的形成，会影响牙龈，形成牙菌斑，进而导致龋齿，尤其是儿童龋齿。通过快速、准确的分子方法对儿童龋齿进行早期诊断，可提高临床病例的诊断能力，从而预防成年后的牙周感染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.