Bazı yabancı otların Plum pox virüs T ile ilgili çalışmalarda indikatör bitki olarak kullanım potansiyelinin belirlenmesi

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi Pub Date : 2024-02-14 DOI:10.18016/ksutarimdoga.vi.1360250

Cemile TEMUR ÇINAR, Elif Dertli̇, Yasin Kaya, Doğan Işik

{"title":"Bazı yabancı otların Plum pox virüs T ile ilgili çalışmalarda indikatör bitki olarak kullanım potansiyelinin belirlenmesi","authors":"Cemile TEMUR ÇINAR, Elif Dertli̇, Yasin Kaya, Doğan Işik","doi":"10.18016/ksutarimdoga.vi.1360250","DOIUrl":null,"url":null,"abstract":"Determination of the potential of some weeds to be used as an indicator plant in studies on Plum pox virus-T \n \nABSTRACT \nThe plum pox virus (PPV) is the cause of sharka, the most destructive viral disease of Prunus species. A unique strain of PPV has so far been identified in Turkey and called as PPV-Turkey (PPV-T). Since viruses are obligate parasitic organisms, they cannot be cultured in artificial nutrient media. Weeds play a significant role in virus ecology and epidemiology as alternate hosts of plant viruses and as food plants for virus vectors. In this study, the potential of some weeds (such as Chenopodium album, Amaranthus retroflexus and Convolvulus arvensis) are indicators plants in experimental studies on PPV-T was investigated. C. album, A. retroflexus plants were inoculated by dusting three leaf on each 3-week-old plant. For each experiment, a homogeneous group of 10 plants grown singly in pots was inoculated PPV-T. Mock- and non-inoculated plants were used as negative controls. Inoculated plants were monitored dayl for symptom development. Following appearance of symptoms on PPV-T inoculated leaves tested by reverse transcription-polymerase chain reaction (RT-PCR). The results obtained from the indicator test showed that when mechanically inoculated of the two out of three plants tested species, C. album (6 out of 10 plants) and A. retroflexus (10 plants) reacted with infection. Initially, this indicator reacted with local symptoms, and later symptoms of systemic infection occurred. \n \nKeywords: Plum pox virus, PPV-Turkey, Herbaceous hosts, \nIndicator plant","PeriodicalId":506958,"journal":{"name":"Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi","volume":"92 6","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18016/ksutarimdoga.vi.1360250","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Determination of the potential of some weeds to be used as an indicator plant in studies on Plum pox virus-T ABSTRACT The plum pox virus (PPV) is the cause of sharka, the most destructive viral disease of Prunus species. A unique strain of PPV has so far been identified in Turkey and called as PPV-Turkey (PPV-T). Since viruses are obligate parasitic organisms, they cannot be cultured in artificial nutrient media. Weeds play a significant role in virus ecology and epidemiology as alternate hosts of plant viruses and as food plants for virus vectors. In this study, the potential of some weeds (such as Chenopodium album, Amaranthus retroflexus and Convolvulus arvensis) are indicators plants in experimental studies on PPV-T was investigated. C. album, A. retroflexus plants were inoculated by dusting three leaf on each 3-week-old plant. For each experiment, a homogeneous group of 10 plants grown singly in pots was inoculated PPV-T. Mock- and non-inoculated plants were used as negative controls. Inoculated plants were monitored dayl for symptom development. Following appearance of symptoms on PPV-T inoculated leaves tested by reverse transcription-polymerase chain reaction (RT-PCR). The results obtained from the indicator test showed that when mechanically inoculated of the two out of three plants tested species, C. album (6 out of 10 plants) and A. retroflexus (10 plants) reacted with infection. Initially, this indicator reacted with local symptoms, and later symptoms of systemic infection occurred. Keywords: Plum pox virus, PPV-Turkey, Herbaceous hosts, Indicator plant

查看原文本刊更多论文

确定一些杂草在梅花痘病毒 T 研究中用作指示植物的潜力

确定一些杂草在梅花痘病毒-T 研究中用作指示植物的潜力 ABSTRACT The plum pox virus (PPV) is the cause of sharka, the most destructive viral disease of Prunus species.迄今为止，已在土耳其发现了一种独特的 PPV 株系，称为 PPV-土耳其（PPV-T）。由于病毒是强制性寄生生物，因此无法在人工营养介质中培养。杂草作为植物病毒的替代宿主和病毒载体的食物植物，在病毒生态学和流行病学中发挥着重要作用。本研究调查了一些杂草（如藜、苋和卷叶芹）在 PPV-T 实验研究中作为指示植物的潜力。通过在每株 3 周龄的植物上撒 3 片叶片的方式接种藜和苋。在每个实验中，都会给单株生长在花盆中的 10 株同质植物接种 PPV-T。模拟植株和未接种植株作为阴性对照。每天监测接种植株的症状发展情况。PPV-T 接种叶片出现症状后，进行反转录聚合酶链反应（RT-PCR）检测。指示剂试验的结果表明，当机械接种三种受试植物中的两种时，C. album（10 株中有 6 株）和 A. retroflexus（10 株）出现了感染反应。最初，该指示剂反应的是局部症状，后来出现了全身感染症状。关键词李痘病毒土耳其 PPV 草本宿主指示植物

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi

自引率

0.00%

发文量