Fingerprint, multi-component content determination, and mechanism in treating Intracerebral Hemorrhage of Tao-He-Cheng-Qi granule

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry Pub Date : 2024-03-01 DOI:10.1016/j.cjac.2024.100367

Zhiyong ZHONG , Riping XIAO , Dequan ZHU , Guowei LI , Wanling ZENG , Dongmei MAI , Jingyi QIU , Xiangdong CHEN , Wenhui LUO , Wencai YE , Dongmei SUN

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引用次数: 0

Abstract

Tao-He-Cheng-Qi granule (THCQ) was derived from Tao-He-Cheng-Qi Decoction, which has been proved to be one of the effective traditional Chinese medicines (TCMs) applied in the treatment of Intracerebral Hemorrhage (ICH). The objective of this investigation was to study the quality control of THCQ and explore its potential mechanism in treating ICH. According to the results, 20 batches of THCQ had similar chromatographic fingerprints. The similarities of chromatograms of the 20 batches were 0.937–0.998 and 15 major chromatographic peaks were identified as the common peaks for the quality control of THCQ. Subsequently, four analytical methods were successfully established to determine 7 index components of THCQ, respectively, which then were applied in 20 batches of THCQ to determine the content of these index components. Besides, animal experiments were conducted to explore the mechanism of THCQ in the treatment of ICH. After a three-day treatment, behavioral changes and brain histopathological evaluations of SD rats showed that THCQ dose-dependently improved the symptoms of rats with ICH. The results of immunohistochemical evaluations showed that THCQ reduced the expressions of TNF-α and IL-1β. The results of protein expressions detected by ELISA revealed that THCQ dose-dependently reduced Notch1 level and increased the expression of VEGFR. Collectively, the mechanisms of THCQ for treating ICH might be closely relevant to the downregulation of Notch and NF-κB and the upregulation of VEGFR. These findings provided insight for studies in investigating the quality control and further clinical application of THCQ.

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道合承气颗粒治疗脑出血的指纹图谱、多组分含量测定及机理研究

道合承气颗粒（THCQ）源自道合承气汤，已被证明是治疗脑出血（ICH）的有效中药之一。本次调查的目的是研究 THCQ 的质量控制，并探索其治疗 ICH 的潜在机制。结果显示，20 个批次的 THCQ 具有相似的色谱指纹图谱。20 个批次的色谱图相似度为 0.937∼0.998，并确定了 15 个主要色谱峰为 THCQ 质量控制的共同峰。随后，成功建立了 4 种分析方法，分别测定了 THCQ 中的 7 种指标成分，并应用这些方法测定了 20 批 THCQ 中这些指标成分的含量。此外，还通过动物实验探讨了THCQ治疗ICH的机理。经过三天的治疗，SD大鼠的行为变化和脑组织病理学评价表明，THCQ剂量依赖性地改善了ICH大鼠的症状。免疫组化评估结果表明，THCQ能降低TNF-α和IL-1β的表达。ELISA检测蛋白表达的结果显示，THCQ剂量依赖性地降低了Notch1的水平，增加了血管内皮生长因子受体（VEGFR）的表达。综上所述，THCQ治疗ICH的机制可能与下调Notch和NF-κB以及上调VEGFR密切相关。这些发现为研究 THCQ 的质量控制和进一步临床应用提供了启示。

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来源期刊

Chinese Journal of Analytical Chemistry 化学-分析化学

CiteScore

3.60

自引率

25.00%

发文量

17223

审稿时长

35 days

期刊介绍： Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.