Sulfur Metabolism in Thiocapsa bogorovii BBS and the Role of HydSL Hydrogenase

IF 1.3 4区生物学 Q4 MICROBIOLOGY

Microbiology Pub Date : 2024-02-07 DOI:10.1134/s002626172360386x

M. Kh. Khasimov, E. P. Petushkova, O. P. Serdyuk, A. A. Tsygankov

引用次数: 0

Abstract—

The specific enzymatic reaction of sulfur reduction to hydrogen sulfide catalyzed by the HydSL hydrogenase of T. bogorovii BBS has so far been observed only in native cells. In this work, we were able to detect the hydrogen-dependent reaction H₂ + S⁰ → H₂S + HS^– + H⁺ in a cell homogenate of these bacteria. This was achieved by destroying the cells in an optimized buffer of high ionic strength. Further purification of the preparation led to a 28-fold increase in its specific activity.

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Thiocapsa bogorovii BBS 的硫代谢和 HydSL 氢酶的作用

摘要迄今为止，我们只在原生细胞中观察到 T. bogorovii BBS 的 HydSL 氢酶催化硫还原为硫化氢的特定酶促反应。在这项工作中，我们能够在这些细菌的细胞匀浆中检测到依赖氢的反应 H2 + S0 → H2S + HS- + H+。这是通过在高离子强度的优化缓冲液中破坏细胞实现的。进一步纯化制备物后，其比活度提高了 28 倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology 生物-微生物学

CiteScore

2.40

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.