Transcriptome Analysis of a Wine Yeast Strain with a Deletion of the CAR1 Arginase Gene

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-02-07 DOI:10.1134/s0026261723603901

A. V. Mardanov, E. A. Vasyagin, E. S. Mardanova, A. V. Beletsky, M. Yu. Shalamitskiy, T. N. Tanashchuk, V. N. Urakov, V. V. Kushnirov, A. L. Rakitin, N. V. Ravin

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Abstract—

Ethyl carbamate, a potential carcinogen, is formed in wine as a result of a chemical reaction between ethanol and urea during fermentation and storage. Using genome editing methods, a modified strain of wine yeast Saccharomyces cerevisiae was constructed, with a deletion of the CAR1 arginase gene, leading to a decrease in the formation of urea from arginine. Transcriptome analysis by high-throughput sequencing identified 738 genes whose transcription level reliably changed more than twofold as a result of deletion of the CAR1 gene. Expression of most genes of the central metabolic pathways in the CAR1 deletion strain was decreased; increased transcription levels were observed for the Map kinase signaling cascades. Among the genes of arginine metabolism pathways, the level of transcription of the ornithine aminotransferase gene CAR2 increased significantly. The wine material obtained using a strain with a deletion of the CAR1 gene under micro-winemaking conditions contained 25% less urea than in the case of using an unmodified strain. The resulting strain of wine yeast, having lower urea levels, may be promising for winemaking.

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缺失 CAR1 精氨酸酶基因的葡萄酒酵母菌株的转录组分析

摘要--氨基甲酸乙酯是一种潜在的致癌物质，在葡萄酒的发酵和储存过程中，乙醇和尿素发生化学反应而形成氨基甲酸乙酯。利用基因组编辑方法，构建了一株改良的葡萄酒酵母--酿酒酵母（Saccharomyces cerevisiae），该菌株缺失了 CAR1 精氨酸酶基因，导致精氨酸生成尿素的能力下降。通过高通量测序对转录组进行分析，发现有 738 个基因的转录水平因 CAR1 基因的缺失而发生了超过两倍的可靠变化。在 CAR1 基因缺失菌株中，大多数中央代谢途径基因的表达量都有所下降；而 Map 激酶信号级联的转录水平则有所上升。在精氨酸代谢途径的基因中，鸟氨酸氨基转移酶基因 CAR2 的转录水平显著增加。在微型酿酒条件下，使用缺失 CAR1 基因的菌株获得的葡萄酒原料中的尿素含量比使用未修饰菌株的情况少 25%。由此产生的葡萄酒酵母菌株尿素含量较低，有望用于葡萄酒酿造。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.