Analysis of sperm cell motion in high viscosity Newtonian and non-Newtonian fluids using a microfluidic channel

IF 2.3 4区工程技术 Q2 INSTRUMENTS & INSTRUMENTATION

Microfluidics and Nanofluidics Pub Date : 2024-02-12 DOI:10.1007/s10404-024-02707-1

Dhiraj B. Puri, Sumit Sunil Kumar, Vadiraj Hemadri, Arnab Banerjee, Siddhartha Tripathi

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Abstract

The migration of sperm cells in a female reproductive tract is responsible for the successful fertilization of the female egg. In this research work, the effect of the surrounding fluids on the motion of sperm cells has been studied using a microfluidic channel. To analyze the motility of sperm, primary motility parameters such as velocity, beat frequency, amplitude, and derived parameters such as linearity, straightness, and wobble have been measured. The results indicate that sperms possess higher progressive motility in non-Newtonian fluids compared to Newtonian fluids in the same viscosity range. The motion of the sperm shows an inverse relationship between the amplitude of the head trajectory and the beat frequency of the flagella. Numerical studies were performed to measure the drag force on these sperm. The trajectories of the flagella, forces acting on sperm, power generated, pulling power, and efficiency of the sperm motion through the fluid medium have been investigated and a relationship between the force and rotation of the flagella has been established. The results show that the flagella also change their shape based on the properties of the surrounding fluid. This study aims to improve our understanding of issues related to infertility diagnosis and help design in-vitro experiments required for sperm separation.

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利用微流体通道分析精子细胞在高粘度牛顿和非牛顿流体中的运动情况

精子细胞在女性生殖道中的迁移是女性卵子成功受精的关键。在这项研究工作中，利用微流体通道研究了周围液体对精子细胞运动的影响。为了分析精子的运动情况，测量了精子的主要运动参数，如速度、跳动频率、振幅，以及衍生参数，如线性、直线度和摆动。结果表明，与相同粘度范围内的牛顿流体相比，精子在非牛顿流体中具有更高的渐进运动能力。精子的运动显示出头部轨迹振幅与鞭毛跳动频率之间的反比关系。对这些精子的阻力进行了数值研究。研究了鞭毛的轨迹、作用在精子上的力、产生的动力、拉力以及精子在流体介质中的运动效率，并确定了鞭毛的力和旋转之间的关系。研究结果表明，鞭毛也会根据周围液体的性质改变形状。这项研究旨在加深我们对不孕症诊断相关问题的理解，并帮助设计精子分离所需的体外实验。

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来源期刊

Microfluidics and Nanofluidics 工程技术-纳米科技

CiteScore

4.80

自引率

3.60%

发文量

审稿时长

2 months

期刊介绍： Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).