High mono-rhamnolipids production by a novel isolate Pseudomonas aeruginosa LP20 from oily sludge: characterization, optimization, and potential application.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Letters in Applied Microbiology Pub Date : 2024-02-01 DOI:10.1093/lambio/ovae016

Chunyan Li, Yujing Wang, Liguo Zhou, Qingfeng Cui, Wenzhe Sun, Junyuan Yang, Han Su, Feng Zhao

{"title":"High mono-rhamnolipids production by a novel isolate Pseudomonas aeruginosa LP20 from oily sludge: characterization, optimization, and potential application.","authors":"Chunyan Li, Yujing Wang, Liguo Zhou, Qingfeng Cui, Wenzhe Sun, Junyuan Yang, Han Su, Feng Zhao","doi":"10.1093/lambio/ovae016","DOIUrl":null,"url":null,"abstract":"<p><p>This study aims to isolate microbial strains for producing mono-rhamnolipids with high proportion. Oily sludge is rich in petroleum and contains diverse biosurfactant-producing strains. A biosurfactant-producing strain LP20 was isolated from oily sludge, identified as Pseudomonas aeruginosa based on phylogenetic analysis of 16S rRNA. High-performance liquid chromatography-mass spectrometry results indicated that biosurfactants produced from LP20 were rhamnolipids, mainly containing Rha-C8-C10, Rha-C10-C10, Rha-Rha-C8-C10, Rha-Rha-C10-C10, Rha-C10-C12:1, and Rha-C10-C12. Interestingly, more mono-rhamnolipids were produced by strain LP20 with a relative abundance of 64.5%. Pseudomonas aeruginosa LP20 optimally produced rhamnolipids at a pH of 7.0 and a salinity of 0.1% using glycerol and nitrate. The culture medium for rhamnolipids by strain LP20 was optimized by response surface methodology. LP20 produced rhamnolipids up to 6.9 g L-1, increased by 116%. Rhamnolipids produced from LP20 decreased the water surface tension to 28.1 mN m-1 with a critical micelle concentration of 60 mg L-1. The produced rhamnolipids emulsified many hydrocarbons with EI24 values higher than 56% and showed antimicrobial activity against Staphylococcus aureus and Cladosporium sp. with inhibition rates 48.5% and 17.9%, respectively. Pseudomonas aeruginosa LP20 produced more proportion of mono-rhamnolipids, and the LP20 rhamnolipids exhibited favorable activities and promising potential in microbial-enhanced oil recovery, bioremediation, and agricultural biocontrol.</p>","PeriodicalId":17962,"journal":{"name":"Letters in Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Letters in Applied Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/lambio/ovae016","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

This study aims to isolate microbial strains for producing mono-rhamnolipids with high proportion. Oily sludge is rich in petroleum and contains diverse biosurfactant-producing strains. A biosurfactant-producing strain LP20 was isolated from oily sludge, identified as Pseudomonas aeruginosa based on phylogenetic analysis of 16S rRNA. High-performance liquid chromatography-mass spectrometry results indicated that biosurfactants produced from LP20 were rhamnolipids, mainly containing Rha-C8-C10, Rha-C10-C10, Rha-Rha-C8-C10, Rha-Rha-C10-C10, Rha-C10-C12:1, and Rha-C10-C12. Interestingly, more mono-rhamnolipids were produced by strain LP20 with a relative abundance of 64.5%. Pseudomonas aeruginosa LP20 optimally produced rhamnolipids at a pH of 7.0 and a salinity of 0.1% using glycerol and nitrate. The culture medium for rhamnolipids by strain LP20 was optimized by response surface methodology. LP20 produced rhamnolipids up to 6.9 g L-1, increased by 116%. Rhamnolipids produced from LP20 decreased the water surface tension to 28.1 mN m-1 with a critical micelle concentration of 60 mg L-1. The produced rhamnolipids emulsified many hydrocarbons with EI24 values higher than 56% and showed antimicrobial activity against Staphylococcus aureus and Cladosporium sp. with inhibition rates 48.5% and 17.9%, respectively. Pseudomonas aeruginosa LP20 produced more proportion of mono-rhamnolipids, and the LP20 rhamnolipids exhibited favorable activities and promising potential in microbial-enhanced oil recovery, bioremediation, and agricultural biocontrol.

查看原文本刊更多论文

从含油污泥中分离出的新型铜绿假单胞菌 LP20 高产单腊肠脂：特性、优化和潜在应用。

本研究旨在分离出能生产高比例单腊肠脂的微生物菌株。含油污泥富含石油，含有多种生物表面活性剂生产菌株。根据 16S rRNA 系统进化分析，从含油污泥中分离出一株生产生物表面活性剂的菌株 LP20，并确定其为铜绿假单胞菌。高效液相色谱-质谱分析结果表明，LP20 产生的生物表面活性剂是鼠李糖脂，主要含有 Rha-C8-C10、Rha-C10-C10、Rha-Rha-C8-C10、Rha-Rha-C10-C10、Rha-C10-C12:1 和 Rha-C10-C12。有趣的是，菌株 LP20 产生了更多的单鼠李糖脂，相对丰度为 64.5%。铜绿微囊藻 LP20 在 pH 值为 7.0、盐度为 0.1%、使用甘油和硝酸盐的条件下生产鼠李糖脂的效果最佳。菌株 LP20 的鼠李糖脂培养基是通过响应面法优化的。LP20 产生的鼠李糖脂最高达 6.9 g-L-1，增加了 116%。LP20 产生的鼠李糖脂能将水表面张力降至 28.1 mN-m-1，临界胶束浓度为 60 mg-L-1。所产生的鼠李糖脂能乳化许多碳氢化合物，其 EI24 值高于 56%，并对金黄色葡萄球菌和克拉多孢子菌具有抗菌活性，抑制率分别为 48.5%和 17.9%。铜绿微囊藻 LP20 产生了更多的单鼠李糖脂，LP20 鼠李糖脂具有良好的活性，在微生物提高石油采收率、生物修复和农业生物防治方面具有广阔的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Letters in Applied Microbiology 工程技术-生物工程与应用微生物

CiteScore

4.40

自引率

4.20%

发文量

225

审稿时长

3.3 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.