Generation of red blood cells from induced pluripotent stem cells.

IF 3.1 3区医学 Q2 HEMATOLOGY

Current Opinion in Hematology Pub Date : 2024-05-01 Epub Date: 2024-02-13 DOI:10.1097/MOH.0000000000000810

Naomi Gunawardena, Stella T Chou

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Abstract

Purpose of review: Human induced pluripotent stem cells (iPSCs) are an attractive source to generate in-vitro-derived blood for use as transfusable and reagent red cells. We review recent advancements in the field and the remaining limitations for clinical use.

Recent findings: For iPSC-derived red blood cell (RBC) generation, recent work has optimized culture conditions to omit feeder cells, enhance red cell maturation, and produce cells that mimic fetal or adult-type RBCs. Genome editing provides novel strategies to improve cell yield and create designer RBCs with customized antigen phenotypes.

Summary: Current protocols support red cell production that mimics embryonic and fetal hematopoiesis and cell yield sufficient for diagnostic RBC reagents. Ongoing challenges to generate RBCs for transfusion include recapitulating definitive erythropoiesis to produce functional adult-type cells, increasing scalability of culture conditions, and optimizing high-density manufacturing capacity.

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用诱导多能干细胞生成红细胞。

综述目的：人类诱导多能干细胞（iPSCs）是一种具有吸引力的来源，可生成体外衍生血液，用作可输血和试剂红细胞。我们回顾了这一领域的最新进展以及在临床应用中仍然存在的局限性：对于 iPSC 衍生红细胞（RBC）的生成，最近的工作已经优化了培养条件，以省略饲养细胞，提高红细胞成熟度，并生成模拟胎儿或成人型红细胞的细胞。基因组编辑为提高细胞产量和创造具有定制抗原表型的设计型红细胞提供了新策略。摘要：目前的方案支持模拟胚胎和胎儿造血的红细胞生产，细胞产量足以用于诊断红细胞试剂。目前在生产输血用红细胞方面面临的挑战包括重现确定性红细胞生成以生产功能性成人型细胞、提高培养条件的可扩展性以及优化高密度生产能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Opinion in Hematology 医学-血液学

CiteScore

6.60

自引率

3.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Hematology is an easy-to-digest bimonthly journal covering the most interesting and important advances in the field of hematology. Its hand-picked selection of editors ensure the highest quality selection of unbiased review articles on themes from nine key subject areas, including myeloid biology, Vascular biology, hematopoiesis and erythroid system and its diseases.