A novel variation of TaGW2-6B increases grain weight without penalty in grain protein content in wheat (Triticum aestivum L.).

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2024-02-13 eCollection Date: 2024-02-01 DOI:10.1007/s11032-024-01455-y

Chan Bi, Chaoxiong Wei, Jinghui Li, Shaozhe Wen, Huanhuan Zhao, Jiazheng Yu, Xintian Shi, Yuan Zhang, Qiaofeng Liu, Yufeng Zhang, Baoyun Li, Mingshan You

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引用次数: 0

Abstract

Yield and quality are two crucial breeding objects of wheat therein grain weight and grain protein content (GPC) are two key relevant factors correspondingly. Investigations of their genetic mechanisms represent special significance for breeding. In this study, 199 F₂ plants and corresponding F_2:3 families derived from Nongda3753 (ND3753) and its EMS-generated mutant 564 (M564) were used to investigate the genetic basis of larger grain and higher GPC of M564. QTL analysis identified a total of 33 environmentally stable QTLs related to thousand grain weight (TGW), grain area (GA), grain circle (GC), grain length (GL), grain width (GW), and GPC on chromosomes 1B, 2A, 2B, 4D, 6B, and 7D, respectively, among which QGw.cau-6B.1, QTgw.cau-6B.1, QGa.cau-6B.1, and QGc.cau-6B.1 shared overlap confidence interval on chromosome 6B. This interval contained the TaGW2 gene playing the same role as the QTLs, so TaGW2-6B was cloned and sequenced. Sequence alignment revealed two G/A SNPs between two parents, among which the SNP in the seventh exon led to a premature termination in M564. A KASP marker was developed based on the SNP, and single-marker analysis on biparental populations showed that the mutant allele could significantly increase GW and TGW, but had no effect on GPC. Distribution detection of the mutant allele through KASP marker genotyping and sequence alignment against databases ascertained that no materials harbored this allele within natural populations. This allele was subsequently introduced into three different varieties through molecular marker-assisted backcrossing, and it was revealed that the allele had a significant effect on simultaneously increasing GW, TGW, and even GPC in all of three backgrounds. Summing up the above, it could be concluded that a novel elite allele of TaGW2-6B was artificially created and might play an important role in wheat breeding for high yield and quality.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01455-y.

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TaGW2-6B 的一个新变异可增加小麦（Triticum aestivum L.）的粒重，而不会降低谷物蛋白质含量。

产量和品质是小麦育种的两个关键目标，其中粒重和谷物蛋白质含量（GPC）是两个关键的相关因子。研究它们的遗传机制对育种具有特殊意义。本研究利用农达3753（ND3753）及其EMS产生的突变体564（M564）的199个F2植株和相应的F2:3家系来研究M564粒重较大和GPC较高的遗传基础。QTgw.cau-6B.1、QGa.cau-6B.1 和 QGc.cau-6B.1 在 6B 染色体上共享重叠置信区间。该区间含有与 QTLs 起相同作用的 TaGW2 基因，因此对 TaGW2-6B 进行了克隆和测序。序列比对发现了两个亲本之间的两个 G/A SNP，其中第七外显子上的 SNP 导致 M564 基因过早终止。基于该 SNP 开发了一个 KASP 标记，对双亲群体的单标记分析表明，突变等位基因能显著提高 GW 和 TGW，但对 GPC 没有影响。通过 KASP 标记基因分型和序列比对数据库，对突变等位基因进行了分布检测，确定自然种群中没有携带该等位基因的材料。随后，通过分子标记辅助回交将该等位基因引入三个不同的品种，结果发现该等位基因对同时提高三个背景中的 GW、TGW 甚至 GPC 均有显著影响。综上所述，可以得出结论：TaGW2-6B 是一个人工创造的新型精英等位基因，它可能在小麦高产优质育种中发挥重要作用：在线版本包含补充材料，见 10.1007/s11032-024-01455-y。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.