Effects of HMGB1/TLR4 on secretion IL-10 and VEGF in human jaw bone-marrow mesenchymal stem cells.

IF 2.2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Applied Oral Science Pub Date : 2024-02-12 eCollection Date: 2024-01-01 DOI:10.1590/1678-7757-2023-0304

Jingjing Kong, Wei Cheng, Lianzhen Chang, Jingyi Yu, Ronglin Wang, Jianli Xie

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引用次数: 0

Abstract

Objective: We aimed to investigate the regulatory effects of HMGB1/TLR4 signaling pathway on the expression of IL-10 and VEGF in human bone marrow mesenchymal stem cells.

Methodology: Human JBMSCs were isolated and cultured. Then, HMGB1 was added into the JBMSCs culture medium, and the protein and mRNA expression levels of IL-10 and VEGF were assessed. Moreover, cells were pretreated with a specific TLR4 inhibitor (TAK-242), and the expression changes of IL-10 and VEGF were compared.

Results: Compared with the control group, exposure to HMGB1 in human JBMSCs up-regulated TLR4, IL-10, and VEGF secretion at both protein and mRNA levels (P<0. 05). In addition, the increased expression of IL-10 and VEGF could be restrained in TAK-242 group compared with the HMGB1 group (P<0.05).

Conclusions: The results indicated that HMGB1 activate TLR4 signaling pathway in Human JBMSCs, which plays a regulatory role in cytokines expression.

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HMGB1/TLR4对人下颌骨骨髓间充质干细胞分泌IL-10和血管内皮生长因子的影响

目的方法：分离并培养人骨髓间充质干细胞（JBMSCs）。方法：分离并培养人骨髓间充质干细胞，在培养基中加入 HMGB1，评估 IL-10 和 VEGF 的蛋白和 mRNA 表达水平。此外，用特异性 TLR4 抑制剂（TAK-242）预处理细胞，比较 IL-10 和 VEGF 的表达变化：结果：与对照组相比，人 JBMSCs 暴露于 HMGB1 会在蛋白和 mRNA 水平上上调 TLR4、IL-10 和 VEGF 的分泌（PConclusions：结果表明，HMGB1 可激活人 JBMSCs 中的 TLR4 信号通路，从而对细胞因子的表达起到调节作用。

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来源期刊

Journal of Applied Oral Science 医学-牙科与口腔外科

CiteScore

4.80

自引率

3.70%

发文量

审稿时长

4-8 weeks

期刊介绍： The Journal of Applied Oral Science is committed in publishing the scientific and technologic advances achieved by the dental community, according to the quality indicators and peer reviewed material, with the objective of assuring its acceptability at the local, regional, national and international levels. The primary goal of The Journal of Applied Oral Science is to publish the outcomes of original investigations as well as invited case reports and invited reviews in the field of Dentistry and related areas.