Absence of new psychoactive substances in wastewater from South Wales, UK, revealed by optimised liquid chromatography-time-of-flight analysis

IF 2.6 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Drug Testing and Analysis Pub Date : 2024-02-15 DOI:10.1002/dta.3659

Bethan Davies, Richard Paul, David Osselton, Richard Evers, Timothy Woolley

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Abstract

New psychoactive substances are produced and marketed to mimic the effects of their illicit counterparts and to attempt to evade drug tests and prosecution. Here, we present the optimisation, validation and application of an analytical method using liquid chromatography–time-of-flight mass spectrometry to detect and quantify 37 new psychoactive substances and illicit substances in wastewater from South Wales, UK, using a targeted analysis method. Sample preparation was performed using solid-phase extraction with Oasis HLB cartridges. The LC separation was performed using a YMC-Triart Phenyl 450 bar column (12 nm, 5 μm, 100 × 3 mm) which provided good separation and resolution for all targeted analytes with a run time of 9 min. The method was validated using the following parameters: sensitivity, selectivity, linearity, accuracy, precision, recovery and matrix effects. The method was then applied to influent wastewater samples collected from two wastewater treatment plants in Wales, UK.

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优化液相色谱-飞行时间分析法揭示英国南威尔士废水中不存在新的精神活性物质。

新精神活性物质的生产和销售是为了模仿其非法同类物质的效果，并试图逃避药物检测和起诉。在此，我们介绍了一种分析方法的优化、验证和应用，该方法采用液相色谱-飞行时间质谱法，以定向分析方法检测和量化英国南威尔士废水中的 37 种新型精神活性物质和非法物质。样品制备采用 Oasis HLB 固相萃取柱。使用 YMC-Triart Phenyl 450 bar 色谱柱（12 nm，5 μm，100 × 3 mm）进行液相色谱分离，该色谱柱对所有目标分析物均具有良好的分离度和分辨率，运行时间为 9 分钟。该方法使用以下参数进行了验证：灵敏度、选择性、线性、准确度、精密度、回收率和基质效应。然后将该方法应用于从英国威尔士的两家污水处理厂采集的废水样品。

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来源期刊

Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

5.90

自引率

24.10%

发文量

191

审稿时长

2.3 months

期刊介绍： As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.