The lncRNA TRG-AS1 promotes the growth of colorectal cancer cells through the regulation of P2RY10/GNA13.

IF 1.6 4区医学 Q3 GASTROENTEROLOGY & HEPATOLOGY

Scandinavian Journal of Gastroenterology Pub Date : 2024-06-01 Epub Date: 2024-02-15 DOI:10.1080/00365521.2024.2318363

Longqing Shi, Baoyang Luo, Linghui Deng, Qi Zhang, Yuanjiu Li, Donglin Sun, Hua Zhang, Lin Zhuang

{"title":"The lncRNA TRG-AS1 promotes the growth of colorectal cancer cells through the regulation of P2RY10/GNA13.","authors":"Longqing Shi, Baoyang Luo, Linghui Deng, Qi Zhang, Yuanjiu Li, Donglin Sun, Hua Zhang, Lin Zhuang","doi":"10.1080/00365521.2024.2318363","DOIUrl":null,"url":null,"abstract":"Background: The lncRNA TRG-AS1 and its co-expressed gene P2RY10 are important for colorectal cancer (CRC) occurrence and development. The purpose of our research was to explore the roles of TRG-AS1 and P2RY10 in CRC progression.Methods: The abundance of TRG-AS1 and P2RY10 in CRC cell lines (HT-29 and LoVo) and normal colon cells FHC was determined and difference between CRC cells and normal cells was compared. LoVo cells were transfected with si-TRG-AS1 and si-P2RY10 constructs. Subsequently, the viability, colony formation, and migration of the transfected cells were analyzed using cell counting kit-8, clonogenicity, and scratch-wound/Transwell® assays, respectively. Cells overexpressing GNA13 were used to further explore the relationship between TRG-AS1 and P2RY10 along with their downstream functions. Finally, nude mice were injected with different transfected cell types to observe tumor formation in vivo.Results: TRG-AS1 and P2RY10 were significantly upregulated in HT-29 and LoVo compared to FHC cells. TRG-AS1 knockdown and P2RY10 silencing suppressed the viability, colony formation, and migration of LoVo cells. TRG-AS1 knockdown downregulated the expression of P2RY10, GNA12, and GNA13, while P2RY10 silencing downregulated the expression of TRG-AS1, GNA12, and GNA13. Additionally, GNA13 overexpression reversed the cell growth and gene expression changes in LoVo cells induced by TRG-AS1 knockdown or P2RY10 silencing. In vivo experiments revealed that CRC tumor growth was suppressed by TRG-AS1 knockdown and P2RY10 silencing.Conclusions: TRG-AS1 knockdown repressed the growth of HT-29 and LoVo by regulating P2RY10 and GNA13 expression.","PeriodicalId":21461,"journal":{"name":"Scandinavian Journal of Gastroenterology","volume":" ","pages":"710-721"},"PeriodicalIF":1.6000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scandinavian Journal of Gastroenterology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/00365521.2024.2318363","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/15 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: The lncRNA TRG-AS1 and its co-expressed gene P2RY10 are important for colorectal cancer (CRC) occurrence and development. The purpose of our research was to explore the roles of TRG-AS1 and P2RY10 in CRC progression.

Methods: The abundance of TRG-AS1 and P2RY10 in CRC cell lines (HT-29 and LoVo) and normal colon cells FHC was determined and difference between CRC cells and normal cells was compared. LoVo cells were transfected with si-TRG-AS1 and si-P2RY10 constructs. Subsequently, the viability, colony formation, and migration of the transfected cells were analyzed using cell counting kit-8, clonogenicity, and scratch-wound/Transwell® assays, respectively. Cells overexpressing GNA13 were used to further explore the relationship between TRG-AS1 and P2RY10 along with their downstream functions. Finally, nude mice were injected with different transfected cell types to observe tumor formation in vivo.

Results: TRG-AS1 and P2RY10 were significantly upregulated in HT-29 and LoVo compared to FHC cells. TRG-AS1 knockdown and P2RY10 silencing suppressed the viability, colony formation, and migration of LoVo cells. TRG-AS1 knockdown downregulated the expression of P2RY10, GNA12, and GNA13, while P2RY10 silencing downregulated the expression of TRG-AS1, GNA12, and GNA13. Additionally, GNA13 overexpression reversed the cell growth and gene expression changes in LoVo cells induced by TRG-AS1 knockdown or P2RY10 silencing. In vivo experiments revealed that CRC tumor growth was suppressed by TRG-AS1 knockdown and P2RY10 silencing.

Conclusions: TRG-AS1 knockdown repressed the growth of HT-29 and LoVo by regulating P2RY10 and GNA13 expression.

查看原文本刊更多论文

lncRNA TRG-AS1通过调控P2RY10/GNA13促进结直肠癌细胞的生长。

背景：lncRNA TRG-AS1及其共表达基因P2RY10对结直肠癌（CRC）的发生和发展具有重要影响。我们的研究旨在探索 TRG-AS1 和 P2RY10 在 CRC 进展中的作用：方法：测定 TRG-AS1 和 P2RY10 在 CRC 细胞系（HT-29 和 LoVo）和正常结肠细胞 FHC 中的含量，并比较 CRC 细胞和正常细胞之间的差异。用 si-TRG-AS1 和 si-P2RY10 构建物转染 LoVo 细胞。随后，分别使用细胞计数试剂盒-8、克隆生成和划痕-创伤/Transwell®试验分析转染细胞的活力、集落形成和迁移。利用过表达 GNA13 的细胞进一步探讨 TRG-AS1 和 P2RY10 之间的关系及其下游功能。最后，给裸鼠注射不同类型的转染细胞，观察体内肿瘤的形成：结果：与FHC细胞相比，TRG-AS1和P2RY10在HT-29和LoVo细胞中明显上调。TRG-AS1敲除和P2RY10沉默抑制了LoVo细胞的活力、集落形成和迁移。TRG-AS1敲除会降低P2RY10、GNA12和GNA13的表达，而P2RY10沉默会降低TRG-AS1、GNA12和GNA13的表达。此外，GNA13的过表达逆转了TRG-AS1敲除或P2RY10沉默诱导的LoVo细胞的细胞生长和基因表达变化。体内实验显示，TRG-AS1敲除和P2RY10沉默抑制了CRC肿瘤的生长：结论：TRG-AS1敲除通过调节P2RY10和GNA13的表达抑制了HT-29和LoVo的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Scandinavian Journal of Gastroenterology 医学-胃肠肝病学

CiteScore

3.40

自引率

5.30%

发文量

222

审稿时长

3-8 weeks

期刊介绍： The Scandinavian Journal of Gastroenterology is one of the most important journals for international medical research in gastroenterology and hepatology with international contributors, Editorial Board, and distribution