Generation of a Commercial-Scale Founder Population of Porcine Reproductive and Respiratory Syndrome Virus Resistant Pigs Using CRISPR-Cas.

IF 3.7 4区 生物学 Q2 GENETICS & HEREDITY
Brian T Burger, Benjamin P Beaton, Matthew A Campbell, Benjamin T Brett, Melissa S Rohrer, Sarah Plummer, Dylan Barnes, Ke Jiang, Sudhir Naswa, Jeremy Lange, Alina Ott, Elizabeth Alger, Gonzalo Rincon, Steven Rounsley, Jeff Betthauser, Namdori R Mtango, Joshua A Benne, Jessica Hammerand, Codie J Durfee, Marisa L Rotolo, Peter Cameron, Alexandra M Lied, Matthew J Irby, David B Nyer, Chris K Fuller, Scott Gradia, Steven B Kanner, Ki-Eun Park, Jerel Waters, Sean Simpson, Bhanu P Telugu, Brianna C Salgado, Alberto Brandariz-Nuñez, Raymond R R Rowland, Matt Culbertson, Elena Rice, A Mark Cigan
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Abstract

Disease resistance genes in livestock provide health benefits to animals and opportunities for farmers to meet the growing demand for affordable, high-quality protein. Previously, researchers used gene editing to modify the porcine CD163 gene and demonstrated resistance to a harmful virus that causes porcine reproductive and respiratory syndrome (PRRS). To maximize potential benefits, this disease resistance trait needs to be present in commercially relevant breeding populations for multiplication and distribution of pigs. Toward this goal, a first-of-its-kind, scaled gene editing program was established to introduce a single modified CD163 allele into four genetically diverse, elite porcine lines. This effort produced healthy pigs that resisted PRRS virus infection as determined by macrophage and animal challenges. This founder population will be used for additional disease and trait testing, multiplication, and commercial distribution upon regulatory approval. Applying CRISPR-Cas to eliminate a viral disease represents a major step toward improving animal health.

利用 CRISPR-Cas 生成猪繁殖与呼吸综合征病毒抗性猪的商业规模创始人种群。
家畜的抗病基因为动物的健康带来了益处,也为农民提供了机会,以满足对廉价优质蛋白质日益增长的需求。此前,研究人员利用基因编辑技术修改了猪的 CD163 基因,证明了该基因对导致猪繁殖与呼吸综合征(PRRS)的有害病毒的抗性。为了最大限度地发挥潜在的效益,这种抗病特性需要在商业上相关的种群中存在,以便猪的繁殖和销售。为了实现这一目标,我们首次建立了一个规模化基因编辑计划,将单一的改良 CD163 等位基因引入四个基因多样化的精英猪品系中。这项工作培育出了健康的猪,经巨噬细胞和动物挑战测定,它们能抵抗 PRRS 病毒感染。这一创始种群将用于更多的疾病和性状测试、繁殖,并在获得监管部门批准后进行商业销售。应用 CRISPR-Cas 技术消灭病毒性疾病是朝着改善动物健康迈出的重要一步。
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来源期刊
CRISPR Journal
CRISPR Journal Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.30
自引率
2.70%
发文量
76
期刊介绍: In recognition of this extraordinary scientific and technological era, Mary Ann Liebert, Inc., publishers recently announced the creation of The CRISPR Journal -- an international, multidisciplinary peer-reviewed journal publishing outstanding research on the myriad applications and underlying technology of CRISPR. Debuting in 2018, The CRISPR Journal will be published online and in print with flexible open access options, providing a high-profile venue for groundbreaking research, as well as lively and provocative commentary, analysis, and debate. The CRISPR Journal adds an exciting and dynamic component to the Mary Ann Liebert, Inc. portfolio, which includes GEN (Genetic Engineering & Biotechnology News) and more than 80 leading peer-reviewed journals.
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