[Development of selective method for mebeverine detection in blood].

Q4 Medicine

Sudebno-Meditsinskaya Ekspertisa Pub Date : 2024-01-01 DOI:10.17116/sudmed20246701134

P S Vikman, O L Balabanova, O Yu Strelova, A N Grebenyuk

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引用次数: 0

Abstract

Objective: To develop the method of biological fluids' sample processing and mebeverine detection to exclude false results' receiving when diagnosing drug intoxication.

Material and methods: The study was carried out using «Mebeverine» (NJCO «North star», Russia) medicine and hydrolysis by enzymes, namely papain, chymotrypsin, trypsin, chymopsin and hyaluronidase, was applied for sample processing. The extractions were analyzed by methods of HPLC-MS/MS on Nexera XR modular liquid chromatograph with LCMS-8050 (Shimadzu) tandem mass spectrometer and GC-MS on gas chromatograph connected with QP-2020 (Shimadzu, Japanese) mono quadrupole mass spectrometer.

Results and conclusion: It has been revealed that using selective method of sample processing, which consists of aqueous phase extraction at pH=2-4 and enzymatic hydrolysis by papain and hyaluronidase during isolation from blood, is required to detect mebeverine in biological liquids. It has been proven that the native mebeverine is hydrolyzed to veratric (3.4-dimethoxybenzoic) acid and mebeverine alcohol at alkalotic pH value of medium. It has been shown that mebeverine extraction is necessary to study using HPLC-MS/MS, which will allow to avoid the native mebeverine degradation in chromatograph injector as with GC-MS method analysis.

查看原文本刊更多论文

[开发血液中甲基比弗林的选择性检测方法]。

目的开发生物液体样本处理和甲基比弗林检测方法，以避免在诊断药物中毒时收到错误结果：研究使用 "甲贝维林"（NJCO "North star"，俄罗斯）药物，并使用木瓜蛋白酶、糜蛋白酶、胰蛋白酶、糜蛋白酶和透明质酸酶等酶进行水解处理样品。提取物在配有 LCMS-8050 （岛津）串联质谱仪的 Nexera XR 模块化液相色谱仪上进行 HPLC-MS/MS 分析，在配有 QP-2020 （岛津，日本）单四极杆质谱仪的气相色谱仪上进行 GC-MS 分析：结果表明，要检测生物液体中的美贝维林，必须采用选择性的样品处理方法，即在 pH=2-4 的水相萃取条件下，用木瓜蛋白酶和透明质酸酶进行酶水解，然后从血液中分离出来。实验证明，在 pH 值为碱性的培养基中，原生的美贝维林会水解为藜芦酸（3.4-二甲氧基苯甲酸）和美贝维林醇。研究还表明，有必要使用 HPLC-MS/MS 方法研究甲基比弗林提取物，这样就可以避免使用 GC-MS 方法分析时，原生甲基比弗林在色谱仪进样器中发生降解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sudebno-Meditsinskaya Ekspertisa Medicine-Pathology and Forensic Medicine

CiteScore

0.60

自引率

0.00%

发文量

期刊介绍： The journal is concerned with the theory and practice of forensic medicine - the problems of thanatology, traumatology, toxicology, serology, forensic obstetrics, forensic dentistry, forensic psychiatry, forensic chemistry, physicotechnical methods of investigation, history of forensic medicine and some problems of criminology and legal laws related to forensic medicine. It publishes original studies by Russian authors, casuistry surveys, abstracts and reviews of Russian and foreign literature, scientific information, reports on scientific conferences.