The secreted feruloyl esterase of Verticillium dahliae modulates host immunity via degradation of GhDFR.

IF 4.8 1区农林科学 Q1 PLANT SCIENCES

Molecular plant pathology Pub Date : 2024-02-01 DOI:10.1111/mpp.13431

Yajuan Wang, Xiwen Liao, Wenjing Shang, Jun Qin, Xiangming Xu, Xiaoping Hu

{"title":"The secreted feruloyl esterase of Verticillium dahliae modulates host immunity via degradation of GhDFR.","authors":"Yajuan Wang, Xiwen Liao, Wenjing Shang, Jun Qin, Xiangming Xu, Xiaoping Hu","doi":"10.1111/mpp.13431","DOIUrl":null,"url":null,"abstract":"<p><p>Feruloyl esterase (ferulic acid esterase, FAE) is an essential component of many biological processes in both eukaryotes and prokaryotes. This research aimed to investigate the role of FAE and its regulation mechanism in plant immunity. We identified a secreted feruloyl esterase VdFAE from the hemibiotrophic plant pathogen Verticillium dahliae. VdFAE acted as an important virulence factor during V. dahliae infection, and triggered plant defence responses, including cell death in Nicotiana benthamiana. Deletion of VdFAE led to a decrease in the degradation of ethyl ferulate. VdFAE interacted with Gossypium hirsutum protein dihydroflavanol 4-reductase (GhDFR), a positive regulator in plant innate immunity, and promoted the degradation of GhDFR. Furthermore, silencing of GhDFR led to reduced resistance of cotton plants against V. dahliae. The results suggested a fungal virulence strategy in which a fungal pathogen secretes FAE to interact with host DFR and interfere with plant immunity, thereby promoting infection.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 2","pages":"e13431"},"PeriodicalIF":4.8000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866084/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular plant pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/mpp.13431","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Feruloyl esterase (ferulic acid esterase, FAE) is an essential component of many biological processes in both eukaryotes and prokaryotes. This research aimed to investigate the role of FAE and its regulation mechanism in plant immunity. We identified a secreted feruloyl esterase VdFAE from the hemibiotrophic plant pathogen Verticillium dahliae. VdFAE acted as an important virulence factor during V. dahliae infection, and triggered plant defence responses, including cell death in Nicotiana benthamiana. Deletion of VdFAE led to a decrease in the degradation of ethyl ferulate. VdFAE interacted with Gossypium hirsutum protein dihydroflavanol 4-reductase (GhDFR), a positive regulator in plant innate immunity, and promoted the degradation of GhDFR. Furthermore, silencing of GhDFR led to reduced resistance of cotton plants against V. dahliae. The results suggested a fungal virulence strategy in which a fungal pathogen secretes FAE to interact with host DFR and interfere with plant immunity, thereby promoting infection.

Abstract Image

查看原文本刊更多论文

大丽轮枝菌分泌的阿魏酰酯酶通过降解 GhDFR 调节宿主免疫力。

阿魏酰酯酶（阿魏酸酯酶，FAE）是真核生物和原核生物许多生物过程的重要组成部分。本研究旨在探究阿魏酰酯酶在植物免疫中的作用及其调控机制。我们从半生植物病原体大丽轮枝菌（Verticillium dahliae）中发现了一种分泌型阿魏酰酯酶 VdFAE。在大丽轮枝菌感染过程中，VdFAE是一种重要的毒力因子，并能触发植物防御反应，包括导致烟草细胞死亡。删除 VdFAE 会导致阿魏酸乙酯降解的减少。VdFAE 与植物先天免疫中的正调控因子--Gossypium hirsutum 蛋白二氢黄烷醇 4 还原酶（GhDFR）相互作用，并促进 GhDFR 的降解。此外，沉默 GhDFR 会降低棉花植株对大丽花病毒的抗性。研究结果表明了一种真菌毒力策略，即真菌病原体分泌 FAE 与宿主 DFR 相互作用，干扰植物免疫，从而促进感染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecular plant pathology 生物-植物科学

CiteScore

9.40

自引率

4.10%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.