Investigation of exosomal tetraspanin profile in sepsis patients as a promising diagnostic biomarker.

IF 2 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biomarkers Pub Date : 2024-03-01 Epub Date: 2024-02-20 DOI:10.1080/1354750X.2024.2319296

Roushka Bhagwan Valjee, Irene Mackraj, Roshila Moodley, Usri H Ibrahim

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引用次数: 0

Abstract

Introduction: Sepsis, a leading cause of mortality globally, has a complex and multifaceted pathophysiology which still requires elucidation. Therefore, this study aimed to analyze and quantify the number of exosomes in sepsis patients from a South African cohort using the ExoView (NanoView Biosciences, Boston, MA) platform.

Methods: Blood samples were collected from black South African patients attending the local Intensive Care Unit (ICU) hospital. Exosomes were isolated and characterize via TEM and CD63 ELISA kits. ExoView was used to determine particle count, particle size distribution and colocalization of different tetraspanin markers.

Results: Exosomal levels in sepsis patients were significantly higher compared to the control group (p < 0.05). Sepsis exosomes showed a homogenous size distribution ranging from 55 to 70 nm. Tetraspanin colocalization analysis revealed that sepsis exosomes have significantly higher CD63/CD9, CD63/CD81 and CD63/CD9/CD81 colocalization percentages than the control group.

Conclusion: This unique tetraspanin colocalization pattern of sepsis exosomes could serve as a potential sepsis biomarker. Further investigations are required to identify sepsis exosomal cargo signatures for further understanding of sepsis pathophysiology in order to develop effective diagnostics and treatments.

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脓毒症患者外泌体Tetraspanin谱作为一种有前途的诊断生物标记物的研究

导言：败血症是全球死亡的主要原因之一，其病理生理学复杂而多面，目前仍有待阐明。因此，本研究旨在利用 ExoView（NanoView Biosciences，波士顿，马萨诸塞州）平台分析和量化来自南非队列的败血症患者体内的外泌体数量：方法：从在当地重症监护室（ICU）医院就诊的南非黑人患者身上采集血液样本。通过 TEM 和 CD63 酶联免疫吸附试剂盒分离和鉴定外泌体。使用ExoView确定粒子数、粒度分布和不同四泛素标记物的共定位：败血症患者的外泌体水平明显高于对照组（p < 0.05）。败血症外泌体的大小分布均匀，从 55 纳米到 70 纳米不等。四泛素共定位分析显示，败血症外泌体的CD63/CD9、CD63/CD81和CD63/CD9/CD81共定位百分比明显高于对照组：结论：脓毒症外泌体独特的四泛素共定位模式可作为潜在的脓毒症生物标志物。为了进一步了解败血症的病理生理学，开发有效的诊断和治疗方法，还需要进一步的研究来确定败血症外泌体货物特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomarkers 医学-毒理学

CiteScore

5.00

自引率

3.80%

发文量

140

审稿时长

3 months

期刊介绍： The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source. Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged: • Biomarkers of disease • Biomarkers of exposure • Biomarkers of response • Biomarkers of susceptibility Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.