Isolation and molecular characterization of lumpy skin disease virus from Tamil Nadu, India during the outbreaks from 2020 to 2022.

IF 1.9 4区医学 Q3 GENETICS & HEREDITY

Virus Genes Pub Date : 2024-04-01 Epub Date: 2024-02-12 DOI:10.1007/s11262-024-02057-8

Manimuthu Prabhu, Shanmugasamy Malmarugan, Sithanandam Rajagunalan, Balakrishnan Govindan, Lakshmi Prasanth Thangavelu, Ganapathi Palanisamy, Revanaiah Yogisharadhya, Kumaragurubaran Karthik

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Abstract

Lumpy skin disease (LSD) caused by LSD virus is a WOAH notifiable, high-impact, transboundary poxviral disease of bovines. The first official report of LSDV in India is from Odisha state during August 2019. Since then, cases have been reported from many states including Tamil Nadu, a Southern state of India. The present study deals with isolation and molecular characterization of LSDV from Tamil Nadu during the period August 2020 to July 2022. LSDV was isolated in embryonated chicken eggs (ECE) and BHK 21 cells and was characterized based on P32, RPO30, and GPCR genes. The phylogenetic analysis revealed that Tamil Nadu isolates from India are closely related to other Indian strains, Kenyan strains and strains from neighboring countries such as Bangladesh, Nepal, and Myanmar confirming the common exotic source for the transboundary spread across borders. The presence of unique signature of amino acid (aa) at specific positions (A11, T12, T34, S99, and P199) in the GPCR sequence confirmed the identity of LSDV. A twelve nucleotide (nt_94-105) insertion and corresponding aa (TILS) at 30-33 position was found in GPCR sequence and characteristic amino acid proline at 98 position (P98) in the RPO30 gene sequence of our isolates was similar to strains from Bangladesh, Nepal, and Myanmar. Further, dissimilarity of our isolates from Neethling like vaccine strains confirms the circulation of virulent filed strains responsible for the outbreaks.

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2020 年至 2022 年疫情爆发期间从印度泰米尔纳德邦分离块状皮肤病病毒并确定其分子特征。

由 LSD 病毒引起的结节性皮肤病（LSD）是一种应由世界动物卫生组织（WOAH）通报的、影响较大的跨境牛痘病毒病。2019 年 8 月，印度奥迪沙邦首次正式报告 LSDV。此后，许多邦都报告了病例，包括印度南部的泰米尔纳德邦。本研究涉及 2020 年 8 月至 2022 年 7 月期间从泰米尔纳德邦分离到的 LSDV 病毒及其分子特征。LSDV 在胚胎鸡卵（ECE）和 BHK 21 细胞中分离，并根据 P32、RPO30 和 GPCR 基因对其进行鉴定。系统发育分析表明，来自印度泰米尔纳德邦的分离株与其他印度毒株、肯尼亚毒株以及来自孟加拉国、尼泊尔和缅甸等邻国的毒株关系密切，这证实了跨境传播的共同外来来源。在 GPCR 序列的特定位置（A11、T12、T34、S99 和 P199）存在独特的氨基酸（aa）特征，证实了 LSDV 的身份。在 GPCR 序列中发现了一个 12 个核苷酸（nt94-105）的插入和 30-33 位的相应 aa（TILS），我们分离的菌株的 RPO30 基因序列中 98 位的特征氨基酸脯氨酸（P98）与孟加拉国、尼泊尔和缅甸的菌株相似。此外，我们的分离物与 Neethling 疫苗菌株的相似性证实了造成疫情爆发的毒力强的备案菌株的流通。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virus Genes 医学-病毒学

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Viruses are convenient models for the elucidation of life processes. The study of viruses is again on the cutting edge of biological sciences: systems biology, genomics, proteomics, metagenomics, using the newest most powerful tools. Huge amounts of new details on virus interactions with the cell, other pathogens and the hosts – animal (including human), insect, fungal, plant, bacterial, and archaeal - and their role in infection and disease are forthcoming in perplexing details requiring analysis and comments. Virus Genes is dedicated to the publication of studies on the structure and function of viruses and their genes, the molecular and systems interactions with the host and all applications derived thereof, providing a forum for the analysis of data and discussion of its implications, and the development of new hypotheses.