Cell Free Microbial DNA Utilization at a Children's Hospital.

IF 0.7 4区医学 Q4 PATHOLOGY

Fetal and Pediatric Pathology Pub Date : 2024-05-01 Epub Date: 2024-02-12 DOI:10.1080/15513815.2024.2315434

Randall Craver, Stephanie Collier, Margot Anderson

引用次数: 0

Abstract

Background: We investigated the utilization of cell free microbial DNA (cfDNA) at a Children's Hospital. Materials and Methods: cfDNA results were assessed regarding the contribution to therapeutic decisions. Results: Of 80 tests on 59 children, 1 test was unevaluable. At least one agent was identified in 45/79 (57%) tests from 34/59 (58.2%) children, 34/79 (43.0%) were negative in 31/59(52.5%) children. Of 45 positive results, 24/79 (30%) were contributory, 15/79 (19%) were diagnostic, 6/79 (7.6%) were diagnostic but diagnosis could have been made with other testing modalities, and 3/79 (3.8%) were diagnostic with minimal previous workup. 21/79 (26.6%) positives were noncontributory. Of 35 negative results, 9/79 (11.4%) were contributory, 26/79 (33.0%) were noncontributory. Efficiency was 30.4-41.8%. cfDNA detected agents not detected by conventional techniques in 22/79 (27.8%), detected different agents in 9/79 (11.4%), and failed to detect agents identified by conventional techniques in 4 (5%). Conclusions: Efficiency of cfDNA was 30.4-41.8.

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儿童医院的无细胞微生物 DNA 利用。

背景：我们调查了一家儿童医院对细胞游离微生物 DNA（cfDNA）的利用情况。材料与方法：评估了 cfDNA 结果对治疗决策的贡献。结果：在对 59 名儿童进行的 80 次检测中，有 1 次检测结果无效。在 34/59 名儿童（58.2%）的 45/79 次（57%）检测中，至少发现了一种病原体；在 31/59 名儿童（52.5%）的 34/79 次（43.0%）检测中，至少发现了一种阴性病原体。在 45 个阳性结果中，24/79（30%）为促成性结果，15/79（19%）为诊断性结果，6/79（7.6%）为诊断性结果，但本可通过其他检测方式做出诊断，3/79（3.8%）为诊断性结果，但之前的检查工作很少。21/79（26.6%）的阳性结果是非致病性的。在 35 个阴性结果中，9/79（11.4%）是促成因素，26/79（33.0%）是非促成因素。22/79（27.8%）的 cfDNA 检测出了常规技术未检测出的病原体，9/79（11.4%）的 cfDNA 检测出了不同的病原体，4（5%）的 cfDNA 检测出了常规技术未检测出的病原体。结论cfDNA 的检测效率为 30.4-41.8。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fetal and Pediatric Pathology 医学-病理学

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Fetal and Pediatric Pathology is an established bimonthly international journal that publishes data on diseases of the developing embryo, newborns, children, and adolescents. The journal publishes original and review articles and reportable case reports. The expanded scope of the journal encompasses molecular basis of genetic disorders; molecular basis of diseases that lead to implantation failures; molecular basis of abnormal placentation; placentology and molecular basis of habitual abortion; intrauterine development and molecular basis of embryonic death; pathogenisis and etiologic factors involved in sudden infant death syndrome; the underlying molecular basis, and pathogenesis of diseases that lead to morbidity and mortality in newborns; prenatal, perinatal, and pediatric diseases and molecular basis of diseases of childhood including solid tumors and tumors of the hematopoietic system; and experimental and molecular pathology.