Optimization of cryoprotectant formulation to enhance the viability of Lactiplantibacillus plantarum NBC99 isolated from human origin.

IF 2 4区生物学 Q3 BIOCHEMICAL RESEARCH METHODS

Preparative Biochemistry & Biotechnology Pub Date : 2024-08-01 Epub Date: 2024-02-12 DOI:10.1080/10826068.2024.2312450

Akif Emre Kavak, İnci Zent, Akın Özdemir, Enes Dertli

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引用次数: 0

Abstract

Freeze drying has been well applied in the preparation of high-efficiency viability probiotic powders. However, the process is generally accompanied by probiotic viability deficiency, which poses a problem for further application. In this study, various kinds of cryoprotectant formulations (skim milk, maltodextrin, and sucrose) were tested to enhance the survival of Lactiplantibacillus plantarum NBC99 after freezing and freeze-drying. An I-optimal experimental design-oriented optimization model was presented to optimize the cryoprotective medium, and the highest cell survival was observed with 25% skim milk, 8.71% maltodextrin, and 1.13% sucrose cryoprotectant as the optimum condition. L. plantarum NBC99 has been a good potential strain for the manufacture of an industrial probiotic, and this research has aimed to investigate the long-term protective effects of optimum cryoprotectant formulations on the viability of bacteria. The results showed the potential value of freeze-dried probiotic L. plantarum NBC99 culture for commercialization.

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优化低温保护剂配方，提高从人体分离的植物乳杆菌 NBC99 的活力。

冷冻干燥法已被广泛应用于制备高效益生菌粉。然而，冷冻干燥过程通常会导致益生菌活力不足，给进一步应用带来问题。本研究测试了各种低温保护剂配方（脱脂奶、麦芽糊精和蔗糖），以提高植物乳杆菌 NBC99 在冷冻和冷冻干燥后的存活率。结果表明，以 25% 脱脂奶、8.71% 麦芽糊精和 1.13% 蔗糖为冷冻保护剂的最佳条件下，细胞存活率最高。植物乳杆菌 NBC99 一直是制造工业益生菌的良好潜在菌株，本研究旨在调查最佳冷冻保护剂配方对细菌存活率的长期保护作用。研究结果表明，冻干植物乳杆菌 NBC99 培养物具有商业化的潜在价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Preparative Biochemistry & Biotechnology 工程技术-生化研究方法

CiteScore

4.90

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： Preparative Biochemistry & Biotechnology is an international forum for rapid dissemination of high quality research results dealing with all aspects of preparative techniques in biochemistry, biotechnology and other life science disciplines.