Hypoxic mesenchymal stem cells (MSCS)-induced interleukin (IL)-10 alleviate systemic lupus erythematosus (SLE) infl ammation through inhibiting interferon (IFN)-gamma production.

Q2 Medicine

Medicinski Glasnik Pub Date : 2024-02-01 DOI:10.17392/1608-23

Yan Wisnu Prajoko, Agung Putra, Ardi Prasetio, Nur Dina Amalina

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引用次数: 0

Abstract

Aim To determine the eff ect of hypoxic mesenchymal stem cells (MSCs) on the interleukin (IL)-10 and interferon (IFN)-gamma in peripheral blood mononuclear cells (PBMCs) from systemic lupus erythematosus (SLE) patients. Methods This study used a post-test control group design. Hypoxic MSCs were isolated and characterized according to their surface marker expression and diff erentiation capacities. PBMCs isolated from SLE patients were divided into three groups: control and two treatment groups. The treatment groups were treated by co-culturing MSCs to PBMCs with a ratio of 1:10 (T1) and 1:1 (T2) for 48 h incubation. Furthermore, IFN-gamma and IL-10 levels were determined by cytometric bead array (CBA) fl ow cytometry. Results Hypoxic MSCs signifi cantly decreased the IFN-gamma levels and increased the IL-10 levels in dose-dependent manner compared to the control group. The highest activity of hypoxic MSCs was noticed in T2 group. Conclusion Hypoxic MSCs- induced IL-10 are important in the control of anti-infl ammatory eff ect on SLE through inhibiting IFN-gamma.

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缺氧间充质干细胞（MSCS）诱导的白细胞介素（IL）-10可通过抑制干扰素（IFN）-γ的产生来缓解系统性红斑狼疮（SLE）的炎症。

目的探讨缺氧间充质干细胞（MSCs）对系统性红斑狼疮（SLE）患者外周血单核细胞（PBMCs）中白细胞介素（IL）-10 和干扰素（IFN）-γ 的影响。方法本研究采用试验后对照组设计。根据缺氧间充质干细胞的表面标记表达和差异分化能力对其进行分离和鉴定。从系统性红斑狼疮患者体内分离出的 PBMC 分成三组：对照组和两个治疗组。治疗组的间充质干细胞与 PBMCs 按 1:10 （T1）和 1:1 （T2）的比例共培养 48 小时。此外，IFN-γ和IL-10水平通过细胞计数珠阵列（CBA）荧光细胞计数法进行测定。结果与对照组相比，缺氧间充质干细胞以剂量依赖的方式显著降低了IFN-gamma的水平，提高了IL-10的水平。T2组缺氧间充质干细胞的活性最高。结论低氧间充质干细胞诱导的IL-10在通过抑制IFN-γ来控制系统性红斑狼疮的抗炎作用中起着重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medicinski Glasnik 医学-医学：内科

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Medicinski Glasnik (MG) is the official publication (two times per year) of the Medical Association of Zenica-Doboj Canton. Manuscripts that present of original basic and applied research from all fields of medicine (general and clinical practice, and basic medical sciences) are invited.