Mutant crtRB1 gene negates the unfavourable effects of opaque2 gene on germination and seed vigour among shrunken2-based biofortified sweet corn genotypes.

IF 2.6 4区生物学 Q2 PLANT SCIENCES

Functional Plant Biology Pub Date : 2024-02-01 DOI:10.1071/FP23179

Brijesh K Mehta, Hema S Chauhan, Sudipta Basu, Anjali Anand, Aanchal Baveja, Rajkumar U Zunjare, Vignesh Muthusamy, Ashok K Singh, Firoz Hossain

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引用次数: 0

Abstract

Sweet corn is one of the most popular vegetables worldwide. However, traditional shrunken2 (sh2 )-based sweet corn varieties are poor in nutritional quality. Here, we analysed the effect of (1) β-carotene hydroxylase1 (crtRB1 ), (2) opaque2 (o2 ) and (3) o2+crtRB1 genes on nutritional quality, germination, seed vigour and physico-biochemical traits in a set of 27 biofortified sh2 -based sweet corn inbreds. The biofortified sweet corn inbreds recorded significantly higher concentrations of proA (16.47μg g-1 ), lysine (0.36%) and tryptophan (0.09%) over original inbreds (proA: 3.14μg g-1 , lysine: 0.18%, tryptophan: 0.04%). The crtRB1 -based inbreds had the lowest electrical conductivity (EC), whereas o2 -based inbreds possessed the highest EC. The o2 +crtRB1 -based inbreds showed similar EC to the original inbreds. Interestingly, o2 -based inbreds also had the lowest germination and seed vigour compared to original inbreds, whereas crtRB1 and o2 +crtRB1 introgressed sweet corn inbreds showed similar germination and seed vigour traits to their original versions. This suggested that the negative effect of o2 on germination, seed vigour and EC is nullified by crtRB1 in the double mutant sweet corn. Overall, o2 +crtRB1 -based sweet corn inbreds were found the most desirable over crtRB1 - and o2 -based inbreds alone.

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在基于缩二基因的生物强化甜玉米基因型中，突变体 crtRB1 基因抵消了缩二基因对萌芽和种子活力的不利影响。

甜玉米是全球最受欢迎的蔬菜之一。然而，传统的基于缩二（sh2）的甜玉米品种营养质量较差。在此，我们分析了(1)β-胡萝卜素羟化酶1（crtRB1）、(2)不透明2（o2）和(3)o2+crtRB1基因对27个基于sh2的生物强化甜玉米近交系的营养品质、发芽率、种子活力和物理生化性状的影响。生物强化甜玉米杂交种的原A（16.47μg g-1）、赖氨酸（0.36%）和色氨酸（0.09%）含量明显高于原始杂交种（原A：3.14μg g-1，赖氨酸：0.18%，色氨酸：0.04%）。以 crtRB1 为基础的杂交种导电率（EC）最低，而以 o2 为基础的杂交种导电率最高。基于 o2+crtRB1 的近交系的导电率与原始近交系相似。有趣的是，与原始近交系相比，以 o2 为基础的近交系的发芽率和种子活力也最低，而以 crtRB1 和 o2+crtRB1 为基础导入的甜玉米近交系的发芽率和种子活力性状与原始近交系相似。这表明，在双突变体甜玉米中，crtRB1 抵消了 o2 对萌芽、种子活力和欧共体的负面影响。总体而言，以 o2+crtRB1 为基础的甜玉米近交系比以 crtRB1- 和 o2 为基础的近交系最理想。

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来源期刊

Functional Plant Biology 生物-植物科学

CiteScore

5.50

自引率

3.30%

发文量

156

审稿时长

1 months

期刊介绍： Functional Plant Biology (formerly known as Australian Journal of Plant Physiology) publishes papers of a broad interest that advance our knowledge on mechanisms by which plants operate and interact with environment. Of specific interest are mechanisms and signal transduction pathways by which plants adapt to extreme environmental conditions such as high and low temperatures, drought, flooding, salinity, pathogens, and other major abiotic and biotic stress factors. FPB also encourages papers on emerging concepts and new tools in plant biology, and studies on the following functional areas encompassing work from the molecular through whole plant to community scale. FPB does not publish merely phenomenological observations or findings of merely applied significance. Functional Plant Biology is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science. Functional Plant Biology is published in affiliation with the Federation of European Societies of Plant Biology and in Australia, is associated with the Australian Society of Plant Scientists and the New Zealand Society of Plant Biologists.