Expression and correlation analysis of silent information regulator 1 (SIRT1), sterol regulatory element-binding protein-1 (SREBP1), and pyroptosis factor in gestational diabetes mellitus.

IF 1.7 4区 医学 Q3 OBSTETRICS & GYNECOLOGY
Ning Han, Xin-Yuan Chang, Zi-Li Yuan, Yi-Zhan Wang
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引用次数: 0

Abstract

Background and aim: Globally, the prevalence of gestational diabetes mellitus (GDM) is rising each year, yet its pathophysiology is still unclear. To shed new light on the pathogenesis of gestational diabetes mellitus and perhaps uncover new therapeutic targets, this study looked at the expression levels and correlations of SIRT1, SREBP1, and pyroptosis factors like NLRP3, Caspase-1, IL-1, and IL-18 in patients with GDM.

Methods: This study involved a comparative analysis between two groups. The GDM group consisted of 50 GDM patients and the control group included 50 pregnant women with normal pregnancies. Detailed case data were collected for all participants. We utilized real-time quantitative PCR and Western Blot techniques to assess the expression levels of SIRT1 and SREBP1 in placental tissues from both groups. Additionally, we employed an enzyme-linked immunosorbent assay to measure the serum levels of SIRT1, SREBP1, and pyroptosis factors, namely NLRP3, Caspase-1, IL-1β, and IL-18, in the patients of both groups. Subsequently, we analyzed the correlations between these factors and clinical.

Results: The results showed that there were significantly lower expression levels of SIRT1 in both GDM group placental tissue and serum compared to the control group (p < 0.01). In contrast, the expression of SREBP1 was significantly higher in the GDM group than in the control group (p < 0.05). Additionally, the serum levels of NLRP3, Caspase-1, IL-1β, and IL-18 were significantly elevated in the GDM group compared to the control group (p < 0.01). The expression of SIRT1 exhibited negative correlations with the expression of FPG, OGTT-1h, FINS, HOMA-IR, SREBP1, IL-1β, and IL-18. However, there was no significant correlation between SIRT1 expression and OGTT-2h, NLRP3, or Caspase-1. On the other hand, the expression of SREBP1 was positively correlated with the expression of IL-1β, Caspase-1, and IL-18, but has no apparent correlation with NLRP3.

Conclusions: Low SIRT1 levels and high SREBP1 levels in placental tissue and serum, coupled with elevated levels of pyroptosis factors NLRP3, Caspase-1, IL-1β, and IL-18 in serum, may be linked to the development of gestational diabetes mellitus. Furthermore, these three factors appear to correlate with each other in the pathogenesis of GDM, offering potential directions for future research and therapeutic strategies.

妊娠期糖尿病中沉默信息调节因子 1(SIRT1)、固醇调节元件结合蛋白-1(SREBP1)和热核因子的表达及相关性分析。
背景和目的:在全球范围内,妊娠糖尿病(GDM)的发病率逐年上升,但其病理生理学仍不清楚。为了揭示妊娠糖尿病的发病机制,或许能发现新的治疗靶点,本研究观察了 GDM 患者体内 SIRT1、SREBP1 以及 NLRP3、Caspase-1、IL-1 和 IL-18 等致热因子的表达水平及相关性:本研究对两组患者进行了比较分析。GDM组包括50名GDM患者,对照组包括50名正常妊娠的孕妇。我们收集了所有参与者的详细病例数据。我们利用实时定量 PCR 和 Western Blot 技术评估了两组孕妇胎盘组织中 SIRT1 和 SREBP1 的表达水平。此外,我们还采用酶联免疫吸附法测定了两组患者血清中 SIRT1、SREBP1 和热解因子(即 NLRP3、Caspase-1、IL-1β 和 IL-18)的水平。随后,我们分析了这些因子与临床的相关性:结果显示,与对照组相比,GDM 组胎盘组织和血清中 SIRT1 的表达水平均明显较低(P P P 结论):胎盘组织和血清中SIRT1水平低、SREBP1水平高,再加上血清中NLRP3、Caspase-1、IL-1β和IL-18等致热因子水平升高,可能与妊娠糖尿病的发生有关。此外,这三个因子似乎在 GDM 的发病机制中相互关联,为未来的研究和治疗策略提供了潜在的方向。
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来源期刊
CiteScore
4.40
自引率
0.00%
发文量
217
审稿时长
2-3 weeks
期刊介绍: The official journal of The European Association of Perinatal Medicine, The Federation of Asia and Oceania Perinatal Societies and The International Society of Perinatal Obstetricians. The journal publishes a wide range of peer-reviewed research on the obstetric, medical, genetic, mental health and surgical complications of pregnancy and their effects on the mother, fetus and neonate. Research on audit, evaluation and clinical care in maternal-fetal and perinatal medicine is also featured.
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