Effect of magnesium oxide nanoparticles and LED irradiation on the viability and differentiation of human stem cells of the apical papilla.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnology Letters Pub Date : 2024-04-01 Epub Date: 2024-02-08 DOI:10.1007/s10529-024-03471-6
Hamed Karkehabadi, Afsaneh Rahmati, Hadiseh Abbaspourrokni, Abbas Farmany, Rezvan Najafi, Rooholah Behroozi, Loghman Rezaei-Soufi, Roshanak Abbasi
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Abstract

Purpose: Currently, regenerative endodontic treatments are gaining more and more attention, and stem cells play a significant role in these treatments. In order to enhance stem cell proliferation and differentiation, a variety of methods and materials have been used. The purpose of this study was to determine the effects of magnesium oxide nanoparticles and LED irradiation on the survival and differentiation of human stem cells from apical papilla.

Methods: The MTT test was used to measure the cell survival of SCAPs that had been exposed to different concentrations of magnesium oxide nanoparticles after 24 and 48 h, and the concentration with the highest cell survival rate was picked for further studies. The cells were classified into four distinct groups based on their treatment: (1) control, which received no exposure, (2) exposure to magnesium oxide nanoparticles, (3) exposure to light emitting diode (LED) irradiation (635 nm, 200 mW/cm2) for 30 s, (4) exposure simultaneously with magnesium oxide nanoparticles and LED irradiation. A green approach was employed to synthesize magnesium oxide nanoparticles. Quantitative real time PCR was used to measure the gene expression of osteo/odontogenic markers such as BSP, DSPP, ALP and DMP1 in all four groups after treatment, and Alizarin red S staining (ARS) was used to determine the osteogenic differentiation of SCAPs by demonstrating the Matrix mineralization.

Results: The highest viability of SCAPs was observed after 24 h in concentration 1 and 10 µg/mL and after 48 h in concentration 1 µg/mL, which were not significantly different from the control group. In both times, the survival of SCAPs decreased with increasing concentration of magnesium oxide nanoparticles (MgONPs). According to the results of Real-time PCR, after 24 and 48 h, the highest differentiation of BSP, DMP1, ALP and DSPP genes was observed in the LED + MgONPs group, followed by MgONPs and then LED, and in all 3 experimental groups, it was significantly higher than control group (P < 0.05). Also, after 24 and 48 h, the density of ARS increased in all groups compared to the control group, and the highest density was observed in the MgONPs + LED and MgONPs groups.

Conclusion: This research concluded that exposure to SCAPs, MgONPs, and LED irradiation has a significant effect on enhancing gene expression of odontogenic/osteogenic markers and increasing matrix mineralization.

Abstract Image

氧化镁纳米颗粒和 LED 照射对根尖乳头人类干细胞活力和分化的影响。
目的:目前,牙髓再生治疗正受到越来越多的关注,而干细胞在这些治疗中发挥着重要作用。为了促进干细胞的增殖和分化,人们使用了多种方法和材料。本研究的目的是确定氧化镁纳米颗粒和 LED 照射对根尖乳头人类干细胞存活和分化的影响:方法:采用 MTT 试验测量暴露于不同浓度氧化镁纳米颗粒 24 小时和 48 小时后 SCAPs 的细胞存活率,并挑选细胞存活率最高的浓度进行进一步研究。根据处理方法将细胞分为四组:(1) 未暴露的对照组;(2) 暴露于氧化镁纳米颗粒的对照组;(3) 暴露于发光二极管(LED)照射(635 nm,200 mW/cm2)30 s 的对照组;(4) 同时暴露于氧化镁纳米颗粒和 LED 照射的对照组。采用绿色方法合成氧化镁纳米粒子。采用实时定量 PCR 法检测四组处理后骨/牙源性标志物(如 BSP、DSPP、ALP 和 DMP1)的基因表达,并采用茜素红 S 染色法(ARS)通过显示基质矿化来确定 SCAPs 的成骨分化情况:结果:浓度为 1 和 10 µg/mL 的 SCAPs 在 24 小时后存活率最高,浓度为 1 µg/mL 的 SCAPs 在 48 小时后存活率最高,与对照组相比无显著差异。在这两个时间段内,随着氧化镁纳米颗粒(MgONPs)浓度的增加,SCAPs 的存活率下降。根据实时 PCR 的结果,24 和 48 h 后,BSP、DMP1、ALP 和 DSPP 基因在 LED + MgONPs 组的分化程度最高,其次是 MgONPs,再次是 LED,且 3 个实验组的分化程度均明显高于对照组(P 结论:LED + MgONPs 组和 LED + MgONPs 组的 BSP、DMP1、ALP 和 DSPP 基因的分化程度最高,其次是 MgONPs,再次是 LED:本研究得出结论:暴露于 SCAPs、MgONPs 和 LED 的照射对提高牙源性/骨源性标志物的基因表达和增加基质矿化有明显作用。
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来源期刊
Biotechnology Letters
Biotechnology Letters 工程技术-生物工程与应用微生物
CiteScore
5.90
自引率
3.70%
发文量
108
审稿时长
1.2 months
期刊介绍: Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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