The Role of Long Noncoding RNA Negative Regulator of Interferon Response in the Regulation of Hantaan Virus Infection.

IF 1.5 4区医学 Q4 IMMUNOLOGY

Viral immunology Pub Date : 2024-01-01 Epub Date: 2024-02-06 DOI:10.1089/vim.2023.0111

Yan Liang, Jiayi Zhan, Hongyan Shi, Wei Ye, Kaixuan Zhang, Jiayu Li, Wei Wang, Pingzhong Wang, Ying Zhang, Jianqi Lian, Xuyang Zheng

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引用次数: 0

Abstract

Hantaan virus (HTNV) is prevalent in Eurasia. It causes hemorrhagic fever with renal syndrome (HFRS). Long noncoding RNAs (lncRNAs) play key roles in regulating innate immunity. Among these, lncRNA negative regulator of interferon response (NRIR) was reported as an inhibitor of several interferon (IFN)-stimulated genes. Our results showed that: NRIR expression was upregulated by HTNV infection in a type I IFN-dependent manner. The expression of NRIR in CD14⁺ monocytes from HFRS patients in acute phase was significantly higher than that in convalescent phase and healthy controls. HTNV infection in some HTNV-compatible cells was promoted by NRIR. NRIR negatively regulated innate immunity, especially IFITM3 expression. Localized in the nucleus, NRIR bound with HNRNPC, and knockdown of HNRNPC significantly weakened the effect of NRIR in promoting HTNV infection and restored IFITM3 expression. These results indicated that NRIR regulates the innate immune response against HTNV infection possibly through its interaction with HNRNPC and its influence on IFITM3.

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长非编码 RNA 干扰素反应负调控因子在调控汉坦病毒感染中的作用

汉坦病毒（HTNV）流行于欧亚大陆。它可导致出血热伴肾综合征（HFRS）。长非编码 RNA（lncRNA）在调节先天性免疫中发挥着关键作用。其中，lncRNA 干扰素反应负调控因子（NRIR）被报道为多个干扰素（IFN）刺激基因的抑制因子。我们的研究结果表明HTNV 感染会以 IFN 依赖性方式上调 NRIR 的表达。急性期 HFRS 患者 CD14+ 单核细胞中 NRIR 的表达明显高于恢复期和健康对照组。NRIR促进了一些与HTNV相容的细胞感染HTNV。NRIR 负向调节先天性免疫，尤其是 IFITM3 的表达。NRIR定位于细胞核，与HNRNPC结合，敲除HNRNPC可显著削弱NRIR促进HTNV感染的作用，并恢复IFITM3的表达。这些结果表明，NRIR可能通过与HNRNPC的相互作用及其对IFITM3的影响来调节抗HTNV感染的先天免疫应答。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Viral immunology 医学-病毒学

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Viral Immunology delivers cutting-edge peer-reviewed research on rare, emerging, and under-studied viruses, with special focus on analyzing mutual relationships between external viruses and internal immunity. Original research, reviews, and commentaries on relevant viruses are presented in clinical, translational, and basic science articles for researchers in multiple disciplines. Viral Immunology coverage includes: Human and animal viral immunology Research and development of viral vaccines, including field trials Immunological characterization of viral components Virus-based immunological diseases, including autoimmune syndromes Pathogenic mechanisms Viral diagnostics Tumor and cancer immunology with virus as the primary factor Viral immunology methods.