Differential sensitivities to gamma radiation of human bladder and testicular tumour cell lines.

C N Parris, C F Arlett, A R Lehmann, M H Green, J R Masters
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引用次数: 33

Abstract

Gamma radiation sensitivities of continuous cell lines from nine human tumours were measured, comparing four derived from transitional cell carcinomas of the bladder with five from non-seminomatous germ cell tumours of the testis. The testicular cells were significantly more radiosensitive than the bladder cells, corresponding to the response to therapy of these tumour types in patients. These observations indicate that radiosensitivity is retained in vitro and is an inherent property of the testicular tumour cells. These gamma radiation sensitivities were compared with those of SV40-transformed fibroblasts derived from a normal individual and one with the heritable disease, ataxia-telangiectasia (A-T). The bladder cells had gamma radiation sensitivities similar to that of the SV40-transformed normal line. The testicular cells were hypersensitive to gamma radiation, although not as sensitive as the SV40-transformed A-T line. A-T cells, unlike those derived from normal individuals, continue to synthesize DNA at a normal rate following radiation exposure, prompting a comparison of the kinetics of DNA synthesis in three bladder and three testicular tumour cell lines. One of the bladder and two testicular lines showed a reduced inhibition when compared to the other tumour cell lines and the SV40-transformed normal line. Thus there was no clear association between DNA synthesis inhibition and radiosensitivity.

人类膀胱和睾丸肿瘤细胞系对γ射线的不同敏感性。
测量了来自9个人类肿瘤的连续细胞系的伽马辐射敏感性,比较了4个来自膀胱移行细胞癌的细胞系和5个来自睾丸非半瘤性生殖细胞肿瘤的细胞系。睾丸细胞明显比膀胱细胞对放射更敏感,这与患者对这些肿瘤类型治疗的反应相对应。这些观察结果表明,放射敏感性在体外保留,是睾丸肿瘤细胞的固有特性。将这些伽马辐射敏感性与来自正常个体和遗传性疾病ataxia-毛细血管扩张症(a-t)患者的sv40转化成纤维细胞进行比较。膀胱细胞对γ辐射的敏感性与转化sv40的正常细胞系相似。睾丸细胞对伽马辐射过敏,但不像sv40转化的A-T细胞系那么敏感。与来自正常人的细胞不同,a - t细胞在辐射暴露后继续以正常速率合成DNA,这促使对三种膀胱和三种睾丸肿瘤细胞系的DNA合成动力学进行比较。与其他肿瘤细胞系和sv40转化的正常细胞系相比,其中一个膀胱细胞系和两个睾丸细胞系的抑制作用降低。因此,DNA合成抑制与放射敏感性之间没有明确的联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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